C7926
Collagenasi
Sigma Blend Type F, ≥2.0 FALGPA units/mg solid
Sinonimo/i:
Clostridiopeptidasi A
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Prodotti consigliati
![N-[3-(2-Furyl)acryloyl]-Leu-Gly-Pro-Ala](/deepweb/assets/sigmaaldrich/product/structures/805/876/96b5fb57-71c8-4c6b-b5d2-fafe7374cd85/640/96b5fb57-71c8-4c6b-b5d2-fafe7374cd85.png)
Origine biologica
Clostridium histolyticum
Livello qualitativo
Forma fisica
powder
caseinase activity
≤10 units/mg solid
Attività specifica
≥2.0 FALGPA units/mg solid
PM
68-130 kDa
Temperatura di conservazione
−20°C
Cerchi prodotti simili? Visita Guida al confronto tra prodotti
Categorie correlate
Applicazioni
Collagenase from Clostridium histolyticum has been used in determining the degradation rate of collagen biomaterials. It may also be used to determine the enzymatic degradation and enzymatic resistance by the liberated L-leucine measurements.
Collagenase from Clostridium histolyticum, or Clostridiopeptidase A, has been used in a study to assess contact dermatitis with clostridiopeptidase A contained in Noruxol ointment. Clostridiopeptidase A has also been used in a study to investigate the early and successful enzymatıc debridement via collagenase application to pinna in a preterm neonate.
The enzyme has been used in the isolation of mast cells from adult mice. This study generated a large number of connective tissue-type mast cells by the culture of murine fetal skin cells. The product has also been used in the in vitro biodegradation study of Bombyx mori silk fibroin fibers and films, by exposing them to the enzyme for varied time periods.
Azioni biochim/fisiol
Effective release of cells from tissue requires the action of collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA)4; β-mercaptoethanol; glutathione, reduced; thioglycolic acid, sodium; and 2,2′-dipyridyl; 8-hydroxyquinoline are known to inhibit the enzyme activity.
La collagenasi è attivata da quattro grammi atomo di calcio per mole di enzima. È inibita da etilen-glicol-bis(beta-amminoetil etere) - acido N, N, N′,N′-tetraacetico, beta-mercaptoetanolo, glutatione, acido tioglicolico e 8-idrossichinolina.
Definizione di unità
Una unità di digestione del collagene (CDU) libera peptidi da collagene proveniente da tendine d′Achille bovino equivalente in colore ninidrina a 1,0 μmole di leucina in 5 ore a pH 7,4 a 37 °C in presenza di ioni calcio. Una unità di idrolisi FALGPA idrolizza 1,0 μmole di furilacriloil-Leu-Gly-Pro-Ala per minuto a 25 °C. Una unità di proteasi neutra idrolizza caseina e produce colore equivalente a 1,0 μmole di tirosina per 5 ore a pH 7,5 a 37 °C. Una unità di clostripaina idrolizza 1,0 μmole of BAEE per minuto a pH 7,6 a 25 °C in presenza di DTT.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Biodegradation of Bombyx mori silk fibroin fibers and films.
Arai T, et al.
Journal of Applied Polymer Science, 91(4), 2383-2390 (2004)
Nobuo Yamada et al.
The Journal of investigative dermatology, 121(6), 1425-1432 (2003-12-17)
We describe a novel culture system for generating large numbers of murine skin-associated mast cells and distinguish their characteristics from bone marrow-derived cultured mast cells. Culture of day 16 fetal skin single cell suspensions in the presence of interleukin-3 and
Properties of collagen and hyaluronic acid composite materials and their modification by chemical crosslinking
Rehakova M, et al.
Journal of Biomedical Materials Research, 30(3), 369-372 (1996)
K Vizárová et al.
Biomaterials, 16(16), 1217-1221 (1995-11-01)
Two kinds of layered atelocollagen materials cross-linked with hexamethylene diisocyanate (HMDIC), starch dialdehyde and glyoxal were enzymatically treated by bacterial collagenase. Evaluating collagenase digestion assay for these material showed progressive differences, particularly in the group of samples cross-linked with HMDIC.
Tyler J Chozinski et al.
Scientific reports, 8(1), 10396-10396 (2018-07-12)
Although light microscopy is a powerful tool for the assessment of kidney physiology and pathology, it has traditionally been unable to resolve structures separated by less than the ~250 nm diffraction limit of visible light. Here, we report on the optimization
Protocolli
To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.