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AB0300

Sigma-Aldrich

Alkaline Phosphatase Blue Membrane Substrate Solution

alkaline phosphatase substrate, solution

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About This Item

Codice UNSPSC:
12352204
eCl@ss:
42010105
NACRES:
NA.83

product name

Alkaline Phosphatase Blue Membrane Substrate Solution, sufficient for 400 mL working substrate

Forma fisica

solution

Livello qualitativo

200

impiego

sufficient for 400 mL working substrate

Temperatura di conservazione

2-8°C

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Descrizione generale

Alkaline phosphatase, a metalloenzyme is a member of ectonucleotidases family. It is present in bile ducts, bone, intestine, placenta and tumors.

Applicazioni

Alkaline Phosphatase Blue Membrane Substrate Solution has been used:
  • to analyse direct alkaline phosphatase (AP) activity
  • to perform alkaline phosphatase assays with colorimetric system
  • to analyse direct alkaline phosphatase (AP) activity of the induced pluripotent stem cells (iPSC) line LND554SV.3

Prior to reaction with alkaline phosphatase, the BCIP® reagent is colorless to faint blue and the Nitro BT reagent is yellow. The two-component mixture develops a bluish-purple product with alkaline phosphatase in membrane-type assays. This substrate is not recommended for ELISA assays.

Stato fisico

A 2 component kit sufficient for preparing 400 mL of working substrate.
Supplied as a two-component buffered alkaline phosphatase substrate containing bromochloroindolyl phosphate (BCIP®) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)

Note legali

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Inhibition of alkaline phosphatase: an emerging new drug target
Al-Rashida M and Iqbal J
Mini-Reviews in Medicinal Chemistry, 15(1), 41-51 (2015)
The Short Isoform of NF-YA Belongs to the Embryonic Stem Cell Transcription Factor Circuitry
Dolfini D, et al.
Stem Cells, 30(11), 2450-2459 (2012)
Generation of a human iPSC line from a patient with Leigh syndrome
Galera T, et al.
Stem Cell Research, 16(1), 63-66 (2016)
Generation of pig iPS cells: a model for cell therapy
Montserrat N, et al.
Journal of Cardiovascular Translational Research, 4(2), 121-130 (2011)
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Articoli

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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