A7981

Anti-Aromatase

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinonimo/i: Anti-ARO, Anti-CYP XIX, Anti-CYP19A1 (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1), Anti-Estrogen Synthase, Anti-P-450 Aromatase
• Codice UNSPSC: 12352203

Proprietà

• Origine biologica: rabbit
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Stato: buffered aqueous solution
• PM: antigen 50-55 kDa
• Reattività contro le specie: human
• Concentrazione: ~1.0 mg/mL
• tecniche: indirect immunofluorescence: 0.5-1 μg/mL using paraformaldehyde fixed HEK-293T cells transfected with Aromatase; western blot: 0.5-1 μg/mL using human placenta tissue lysate
• N° accesso UniProt: P11511
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... CYP19A1(1588)

Descrizione

Descrizione generale

The enzyme aromatase is a member of P450 cytochrome family. Aromatase catalyzes three oxidation reactions of androstenedione A that lead to aromatization of androgens and subsequent synthesis of estrogen. The enzymatic complex of aromatase is a heterocomplex of cytochrome 450 aromatase and NADPH cytochrome P450 reductase.[1][2] The tissues that produce aromatase are placenta, ovary, brain, bone, adipose tissue, skin and testis.[3] The aromatase enzyme complex is present in the smooth endoplasmic reticulum of cells of these tissues.[4] High levels of aromatase and estrogen are produced by the breast cancer cells. Selective aromatase inhibitors are developed to control the recurrence and survival of hormone-dependent breast cancer tissue[1]
Anti-Aromatase specifically recognizes human aromatase (50-55 kDa).

Applicazioni

Anti-Aromatase may be used for immunoblotting at a working concentration of 0.5-1 μg/ml using lysate of human placenta tissue. The antibody has also been used for immunoblotting using whole extracts[5] or nuclear extracts[6] of human primary trophoblasts. For immunofluorescence, a working concentration of 0.5-1 μg/ml is recommended.

Descrizione del bersaglio

Aromatase is part of the cytochrome p450 family of monooxygenases. It is involved in the production of estrogen and found localized to the endoplasmic reticulum of multiple cell types. [7]

Stato fisico

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)