After each separation, elute any reversibly bound material either with a high ionic strength solution (e.g. 1 M NaCl in buffer) or by increasing pH. Regenerate the media by washing with at least 5 bed volumes of buffer, or until the column effluent shows stable conductivity and pH values.
A25120
DEAE–Sephadex® A-25 chloride form
Sinonimo/i:
DEAE Sephadex®, Sephadex A 25,2-(diethylamino)ethylether, Diethylaminoethyl Sephadex®
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Scegli un formato
10 G
139,00 €
50 G
404,00 €
Scegli un formato
Cambia visualizzazione
10 G
139,00 €
50 G
404,00 €
About This Item
Prodotti consigliati
Stato
powder
Livello qualitativo
tecniche
activity assay: suitable
Matrice
cross-linked dextran
Gruppo funzionale matrice
functional group
Dimensioni delle microsfere
40-125 μm (dry)
Dimensione pori
~30,000 Da exclusion limit
pH di lavoro
2-9
Capacità
3-4 meq/g ion exchange capacity
Compatibilità
mode of use mixed weak and strong anion exchange chromatography
Descrizione generale
The Sephadex® ion exchangers contain a cross-linked dextran matrix with functional groups. These groups are linked by stable ether linkages to the glucose units in the matrix. DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins.
A25120-100G′s updated product number is GE17-0170-01
A25120-500G′s updated product number is GE17-0180-02
A25120-100G′s updated product number is GE17-0170-01
A25120-500G′s updated product number is GE17-0180-02
Applicazioni
DEAE-Sephadex® A-25 has been used:
- to form DEAE-Sephadex A-25 (formate form) for glucosinolates (GLS) extraction using horseradish plants
- in the estimation of GLS content in Chinese kale sprouts
- production and purification of endozepine from astrocytes
- to identify and label an insulin-activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients
Caratteristiche e vantaggi
- Weak anion exchanger
- Suitable for batch techniques
- High binding capacities
- Non-reactive to commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride
Note legali
DEAE Sephadex is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva
Sostituito da
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Scegli una delle versioni più recenti:
Certificati d'analisi (COA)
Lot/Batch Number
Non trovi la versione di tuo interesse?
Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Dong-Lin Wang et al.
Journal of agricultural and food chemistry, 50(2), 262-265 (2002-01-10)
Among 10 lipases tested, Candida rugosa lipase exhibited the best ability to catalyze the resolution of dl-menthol in organic solvent. The lipase was immobilized on different carriers, and the experiment was carried out with different acyl donors. The high yield
DEAE-silica gel and DEAE-controlled porous glass as ion exchangers for the isolation of glycolipids.
S K Kundu
Methods in enzymology, 72, 174-185 (1981-01-01)
M J Hollingsworth et al.
Molecular and cellular biology, 6(4), 1058-1064 (1986-04-01)
A requisite step in the biosynthesis of tRNA is the removal of 5' leader sequences from tRNA precursors. We have detected an RNase P activity in yeast mitochondrial extracts that can carry out this reaction on a homologous precursor tRNA.
Scott N Boyle et al.
Biochemistry, 46(41), 11614-11620 (2007-09-26)
Abl family kinases have been implicated in the regulation of cell morphogenesis and migration, but the molecular mechanisms through which they operate are not fully elucidated. We applied the bump-hole technique, pioneered by Shokat and colleagues, to identify direct substrates
Shu-Kui Wang et al.
Zhonghua nan ke xue = National journal of andrology, 8(3), 173-177 (2002-12-14)
To establish and evaluate the anti-human seminal plasma phospholipase A2 (PLA2) monoclonal antibody (McAb). After having been separated and purified from human seminal plasma by PEG precipitation, Sephacryl S-300 column chromatography, DEAE-Sephadex A-25 column chromatography and HA column chromatography, PLA2
-
How should I regenerate Product A25120, DEAE-Sephadex® A-25 chloride form?
1 risposta-
Utile?
-
-
What is the Department of Transportation shipping information for this product?
1 risposta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Utile?
-
-
How should I store Product A25120, DEAE-Sephadex® A-25 chloride?
1 risposta-
Unused media can be stored in the container at +4 to +30 °C. Ensure that the screw-top is fully tightened. Packed columns should be equilibrated in working buffer containing 20% ethanol to prevent microbial growth.
Utile?
-
-
How do I equilibrate Product A25120, DEAE-Sephadex® A-25 chloride form?
1 risposta-
After packing, and before a chromatographic run, equilibrate with working buffer by washing with at least 5 bed volumes.
Utile?
-
-
What is the chemical stability of Product A25120, DEAE-Sephadex® A-25 chloride form?
1 risposta-
According to the manufacturer, this is compatible with all commonly used aqueous buffers, 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride and 70% ethanol.
Utile?
-
Filtri attivi
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.
