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93017

Sigma-Aldrich

SSC Buffer 20x

for Northern and Southern blotting, solution

Sinonimo/i:

20x SSC

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About This Item

Codice UNSPSC:
12161700
NACRES:
NA.25

Grado

for molecular biology

Livello qualitativo

200

Nome Commerciale

BioReagent

Stato

solution

tecniche

Southern blotting: suitable

Impurezze

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

pH

7.0±0.2

Densità

1.16 g/mL at 20 °C

Temperatura di conservazione

room temp

Descrizione generale

SSC Buffer 20x Concentrate is a standard reagent in Southern and Northern blotting procedures. This concentrated formula can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Applicazioni

Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

Caratteristiche e vantaggi

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations
  • Easily diluted to any concentration needed

Componenti

SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves

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Certificati d'analisi (COA)

Lot/Batch Number
BCCL6351
BCCK4994
BCCH4851
BCCG6608
BCCG1453

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Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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