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49940

Millipore

ONPG Disks

suitable for microbiology

Sinonimo/i:

β-Galactosidase Test Disks, 2-Nitrophenyl-β-D-galactopyranoside Disks

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About This Item

Codice UNSPSC:
41171621
NACRES:
NA.85

agenzia

according to GB 4789.4-2016

Nome Commerciale

BioChemika

Durata

limited shelf life, expiry date on the label

tecniche

microbe id | specific enzyme detection: suitable

applicazioni

environmental
food and beverages
microbiology

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Compatibilità

Escherichia coli
bacteria

Descrizione generale

ONPG Disks are used to detect the presence of β-galactosidase, an enzyme found in lactose-fermenting organisms. Lactose utilization depends upon two enzymes: β-galactoside permease, which catalyzes transport of lactose into the cell, and β-galactosidase, which breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides including the ONPG (o-nitrophenyl-β-D-galactopyranose) substrate. ONPG hydrolysis results in the release of galactose, and the yellow chromogenic compound, o-nitrophenol. The test substrate, ONPG, does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period even for late lactose fermenters.

Confezionamento

Sterile filter paper discs (50 pcs, diameter 6 mm) impregnated with o-nitrophenyl-β-D-galactopyranose

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
BCCL6201
BCCM1925
BCCK3268
BCCJ4470
BCCG9930

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