16669

Magenta-Gal

reagent for selection of recombinant bacterial clones

• Sinonimo/i: 5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside, Mag-Gal
• Formula empirica (notazione di Hill): C₁₄H₁₅BrClNO₆
• Numero CAS: 93863-88-8
• Peso molecolare: 408.63
• Beilstein: 1552602
• Numero MDL: MFCD00210022
• Codice UNSPSC: 12352200
• ID PubChem: 329751317

Proprietà

• Grado: for molecular biology
• Livello qualitativo: 100
• Sterilità: non-sterile
• Saggio: ≥98.0% (HPLC)
• Forma fisica: powder
• Solubilità: DMF: soluble; DMSO: soluble
• Compatibilità: suitable for β-galactosidase test
• Temperatura di conservazione: −20°C
• Stringa SMILE: OC[C@H]1O[C@@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11-,12+,13-,14-/m1/s1; CHRVKCMQIZYLNM-RKQHYHRCSA-N

Descrizione

Descrizione generale

Magenta-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Magenta-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Applicazioni

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Also suitable for use immunoblotting and immunocytochemical assays.

Principio

When Magenta-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Magenta-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 13 - Non Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)