LMDH-RO

L-Malate Dehydrogenase (L-MDH)

from pig heart (mitochondrial)

• Classificazione EC (Enzyme Commission): 1.1.1.37 (BRENDA, IUBMB)
• Codice UNSPSC: 12352202

Proprietà

• Origine biologica: Porcine heart (mitochondrial)
• Livello qualitativo: 100
• Forma fisica: solution; suspension
• Attività specifica: ~1200 units/mg protein (At 25 °C with oxaloacetate as the substrate.)
• Confezionamento: pkg of 1 mL (10127248001 solution); pkg of 1 mL (10127256001 suspension); pkg of 5 mL (10127914001suspension)
• Produttore/marchio commerciale: Roche
• tecniche: activity assay: suitable
• Colore: white
• pH ottimale: 7.4-7.5(reduction of oxaloacetate); 9.2-9.5(oxidation of malate)
• Solubilità: water: miscible
• Compatibilità: suitable for Western blot
• N° accesso NCBI: NM_001244153.1
• N° accesso UniProt: P00346
• applicazioni: life science and biopharma
• Attività estranea: Fumarase <0.01%; GIDH 0.001%; GOT 0.001%; GPT 0.00185%; LDH <0.01%; NADH oxidase <0.001%
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• Informazioni sul gene: Porcine ... MDH2(397039)

Descrizione

Descrizione generale

L-malate:NAD⁺ oxidoreductase
The L-malate dehydrogenase enzyme is a nuclear gene product that is synthesized with a 24-residue amino-terminal signal peptide. This peptide is proteolytically cleaved during the translocation of the enzyme to the mitochondrial matrix.

Applicazioni

The enzyme L-malate dehydrogenase from pig heart has been used to measure PEPCK (phosphoenolpyruvate carboxykinase) activity. The oxaloacetate, produced by PEPCK, is reduced to malate via the oxidation of NADH, which in turn is measured at 340 nm using a spectrophotometer. The enzyme has also been used to measure oxaloacetate by measuring the reduction in NADH spectroscopically at 340 nm.

Azioni biochim/fisiol

The enzyme L-malate dehydrogenase from pig heart catalyzes the oxidation of L-malate to oxaloacetate. The enzyme is an NAD-dependent mitochondrial dehydrogenase that functions in the tricarboxylic acid cycle. It is a component of the malate-aspartate shuttle that transports reducing equivalents across the inner mitochondrial membrane in the form of malate.

Qualità

Contaminants: <0.002% GOT, <0.01% fumarase and LDH, each luM each, <0.02% HK, <0.002% PGI

Stato fisico

Solution in 50% glycerol (v/v), pH approximately 7

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Nota sulla preparazione

Activator: – phosphate
– arsenate
– Mg²⁺
– Zn²⁺

Altre note

For life science research only. Not for use in diagnostic procedures.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): No data available
• Punto d’infiammabilità (°C): No data available