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11333089001

Roche

Anti-Digoxigenin

from sheep

Sinonimo/i:

anti-digoxigenin, digoxigenin

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About This Item

Codice UNSPSC:
12352203

Origine biologica

sheep

Livello qualitativo

100

Coniugato

unconjugated

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

lyophilized

Confezionamento

pkg of 200 μg

Produttore/marchio commerciale

Roche

Isotipo

IgG

Temperatura di conservazione

2-8°C

Descrizione generale

Digoxigenin is a hapten which is used in labeling of nucleic acids and in detection systems.
Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes. It allows faster detection, is less hazardous and has an increased shelf life.

Specificità

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.

Applicazioni

Anti-Digoxigenin has been used in DNA tethering. It has been used to attach DNA molecule to the glass surface of the flow cell.
Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled compounds using:
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Western blot

Azioni biochim/fisiol

In the presence of Na+, Mg2+ and ATP, digoxigenin inhibits sodium pumps.

Nota sulla preparazione

Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
  • ELISA: for coating: 2 to 4 μg/ml
  • Immunohistocytochemistry: 0.5 to 2 μg/ml
  • In situ hybridization: 0.2 to 0.4 μg/ml
  • Western blot: 0.5 to 2 μg/ml

Working solution: For coating applications: PBS (phosphate buffered saline), pH 7.4
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption.

Ricostituzione

Add 1 ml PBS to a final concentration of 200 μg/ml.

Risultati analitici

No cross reactivity with other steroids, such as human estrogens e.g., estradiol or androgens e.g., testosterone.

Altre note

For life science research only. Not for use in diagnostic procedures.

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Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H317

Classi di pericolo

Skin Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

does not flash

Punto d’infiammabilità (°C)

does not flash

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Affinity Labeling of a Sulfhydryl Group in the Cardiacglycoside Receptor Site of Na+/K+?ATPase by N?Hydroxysuccinimidyl Derivatives of Digoxigenin.
Antolovic R, et al.
European Journal of Biochemistry, 227(1?2), 61-67 (1995)
Juliette Salvaing et al.
PloS one, 7(5), e38156-e38156 (2012-06-14)
In the mouse zygote, DNA methylation patterns are heavily modified, and differ between the maternal and paternal pronucleus. Demethylation of the paternal genome has been described as an active and replication-independent process, although the mechanisms responsible for it remain elusive.
Pengyu Hao et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(30), 17775-17784 (2020-07-17)
DNA mismatch repair (MMR), the guardian of the genome, commences when MutS identifies a mismatch and recruits MutL to nick the error-containing strand, allowing excision and DNA resynthesis. Dominant MMR models posit that after mismatch recognition, ATP converts MutS to
Jonathan Silver et al.
Biosensors & bioelectronics, 63, 117-123 (2014-07-30)
We describe a proof-of-principle, immune sandwich assay in which immune complexes link micron-size beads via DNA tethers to a sensor surface. The number of tethered beads, counted using low-magnification microscopy, provides a measure of the concentration of analyte. The prototype
Nonradioactive labeling of probe with digoxigenin by polymerase chain reaction.
Lion T and Haas O A
Analytical Biochemistry, 188(2), 335-337 (1990)
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Articoli

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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