MAB1965

Anti-Integrin beta 1 (CD29) Antibody

CHEMICON^®, mouse monoclonal, JB1A (J10)

• Sinonimo/i: CD29
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Nome del prodotto: Anti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10), ascites fluid, clone JB1A (J10), Chemicon^®
• Origine biologica: mouse
• Livello qualitativo: 100; 300
• Forma dell’anticorpo: ascites fluid
• Tipo di anticorpo: primary antibodies
• Clone: JB1A (J10), monoclonal
• Reattività contro le specie: human
• Produttore/marchio commerciale: Chemicon^®
• tecniche: flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG1
• N° accesso NCBI: NM_002211.2; NM_033666.1; NM_033667.1; NM_033668.1; NM_033669.1; NM_133376.1
• N° accesso UniProt: P05556
• Condizioni di spedizione: dry ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ITGB1(3688)

Descrizione

Descrizione generale

The primary function of the integrin family is to mediate cell-cell and cell-matrix adhesion interaction. CD29 is ubiquitous, with broad tissue distribution, but is not expressed on erythrocytes and is expressed only weakly on granulocytes. Integrins play an important role in cell adhesion and migration, and their normal function is critical in the induction and maintenance of cell differentiation. Loss or downregulation of CD29 has been proposed to be one of the general pathways through which carcinoma cells may acquire a more invasive and differentiated phenotype. Furthermore, loss or downregulation of integrin occurs relatively frequently in poorly differentiated colorectal and breast carcinomas as determined by immunohistochemistry.

Specificità

Reacts with the human integrin beta 1. Note: integrin beta 1 is expressed in all tissues/cell types except erythrocytes. The epitope recognized by JB1a was recently mapped to residues 82-87 of the mature beta 1 integrin chain. In this study Mn++ was found to inhibit binding of JB1a both to mature beta 1 integrin and to a synthetic peptide corresponding to residues 82-87{Ni, 1998}.

Immunogeno

Epitope: a.a. 82-87

Purified beta 1 integrin and Jurkat cells

Applicazioni

Inhibition of Jurkat binding to collagen and fibronectin:
1:100 Note: epitope is trypsin sensitive; cells should be prepared with PBS + EDTA (2mM) or other non-enzymatic methods. Blocking will not occur with trypsinized cells with JB1A.

Immunoprecipitation: 1:250-500

Western blotting (reducing, 115 kDa; non-reducing, 130 kDa): 1:1000-2000

Note: binding of antibody may be inhibited by magnesium ion concentrations exceeding 0.5mM.

Immunocytochemistry/Immunohistochemistry: JB1A can be used on unfixed cells in flow cytometry and culture {Akimov, 2001; Blood 98(5):1567-1576}. Often times fixing with 2-3% PFA for 5 minutes after primary antibody incubation will assist in obtain strong signals since it fixes the primary antibodies to the tissues.

FACS: JB1A has been primarily used against live cells at 1:200-1:500 followed by goat anti-mouse conjugated secondaries

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

This Anti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10) is validated for use in FC, IH, IP, FUNC, WB for the detection of Integrin β1.

Descrizione del bersaglio

88 kDa

Stato fisico

Ascites fluid containing no preservatives.

Unpurified

Stoccaggio e stabilità

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Risultati analitici

Control
Human lung, liver, and skeletal muscle tissues

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable