69053
BLR(DE3) Competent Cells - Novagen
Escherichia coli, rod shaped
Sinonimo/i:
Competent cells for cloning
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About This Item
Codice UNSPSC:
41106202
NACRES:
NA.81
Prodotti consigliati
product name
BLR(DE3) Competent Cells - Novagen, BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences.
Origine biologica
Escherichia coli
Livello qualitativo
Produttore/marchio commerciale
Novagen®
Condizioni di stoccaggio
OK to freeze
avoid repeated freeze/thaw cycles
Modalità di accrescimento
adherent or suspension
Morfologia
rod shaped
tecniche
microbiological culture: suitable
Trasformazione cellulare
transformation efficiency: >2×106 cfu/μg
Condizioni di spedizione
dry ice
Temperatura di conservazione
−70°C
Categorie correlate
Descrizione generale
Genotype: F-ompT hsdSB(rB- mB-) gal lac ile dcm Δ(srl-recA)i306::Tn10 (tetR)(DE3)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
BLR is a recA- derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences or whose products may cause the loss of the DE3 prophage (1, 2).
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences.
Componenti
0.4 ml1 mlComponent
•2 × 0.2 ml5 × 0.2 mlBLR(DE3) Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•2 ng2 ngTest Plasmid
•2 × 0.2 ml5 × 0.2 mlBLR(DE3) Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•2 ng2 ngTest Plasmid
Attenzione
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Altre note
1. A. Roca (U. of Wisconsin), personal
communication.
2. Studier, F. W. (1991) J. Mol. Biol. 219,
37-44.
communication.
2. Studier, F. W. (1991) J. Mol. Biol. 219,
37-44.
Note legali
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Certificati d'analisi (COA)
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