648315

Tris(idrossimetil)aminometano

Sterile Solution. Tris Buffer, 100 mM, pH 7.4, Molecular Biology Grade

• Sinonimo/i: 2-ammino-2-(idrossimetil)-1,3-propanediolo, THAM, Tris base, Trometamolo
• Formula condensata: NH₂C(CH₂OH)₃
• Numero CAS: 77-86-1
• Peso molecolare: 121.14
• Beilstein: 741883
• Numero MDL: MFCD00004679
• Codice UNSPSC: 12161705
• NACRES: NA.25

Proprietà

• Nome del prodotto: Tris(idrossimetil)aminometano,
• Grado: Molecular Biology
• Livello qualitativo: 100
• Descrizione: Merck USA index - 14, 9772
• Stato: liquid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Concentrazione: 100 mM
• Colore: clear colorless
• Intervallo di pH utile: 7-9
• pKa (25 °C): 8.1
• P. ebollizione: 219-220 °C/10 mmHg (lit.)
• Punto di fusione: 167-172 °C (lit.)
• Attività estranea: DNases, RNases, none detected
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 10-30°C
• Stringa SMILE: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2; LENZDBCJOHFCAS-UHFFFAOYSA-N

Descrizione

Descrizione generale

Sterile solution. Widely used biological buffer. Suitable for DNA and RNA applications. Useful in the pH range of 7.0-9.0.

Widely used biological buffer. Prepared with diethylpyrocarbonate-treated water for molecular biology applications. Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Applicazioni

• Structural Characterization of Toxicologically Relevant Cd(2+)-L-Cysteine Complexes.: This study utilized Tris buffer, 100 mM, pH 7.4, for the structural analysis of cadmium-cysteine complexes, providing insights into their biochemical behavior and toxicological impacts (Gautam et al., 2023).


• Cation and buffer specific effects on the DNA-lipid interaction.: The research employed Tris buffer, 100 mM, pH 7.4, to explore the specific effects of cations and buffers on DNA-lipid interactions, highlighting its importance in biophysical and biochemical studies (Mura et al., 2023).


• Anti-inflammatory and antibacterial activities of cerium-containing mesoporous bioactive glass nanoparticles for drug-free biomedical applications.: The research utilized Tris buffer, 100 mM, pH 7.4, in the preparation of cerium-containing nanoparticles, demonstrating their potential in biomedical applications without the need for drugs (Kurtuldu et al., 2021).

Attenzione

Toxicity: Irritant (B)

Stato fisico

A sterile, 100 mM solution, pH 7.4.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable