DMF: 0.5 mg/mL (Small yellow particles may be visible.) DMSO: 0.5 mg/mL
Fluorescenza
λex ~378 nm λem ~503 nm
Temperatura di conservazione
2-8°C
Descrizione generale
A cell-permeable imidazoline-2-thione based compound that acts as a direct, rapid, selective and sensitive fluorescence turn-on detection probe for hypochlorous acid (HOCl; detection limit of 71 nM). Efficiently reacts with hypochlorite to yield fluorescent imidazolium ions, with good water solubility and photostability. Generates negligible fluorescence emission with several other ROS/RNS (H2O2, TBHP, NO•, ROO•, ONOO- and HO•. Shown to efficiently visualize and detect stimulants triggered endogenous HOCl generation in RAW 264.7 macrophages co-cultured with HeLa cells. Dose-dependently images HOCl levels by two-photon fluorescence microscopy in RAW 264.7 cells and in rat hippocampal slices (depth of >100 µm).
A cell-permeable imidazoline-2-thione based compound that acts as a direct, rapid, selective and sensitive fluorescence turn-on detection probe for hypochlorous acid (HOCl; detection limit of 71 nM). Efficiently reacts with hypochlorite to yield fluorescent imidazolium ions, with good water solubility and photostability. Generates negligible fluorescence emission with several other ROS/RNS (H2O2, TBHP, NO·, ROO·, ONOO− and HO·). Shown to efficiently visualize and detect stimulants triggered endogenous HOCl generation in RAW 264.7 macrophages co-cultured with HeLa cells. Also enables to monitor HOCl levels by two-photon image in RAW 264.7 cells and in rat hippocampal slices (depth of >100 µm) in a dose-dependent manner.
Azioni biochim/fisiol
Cell permeable: yes
Primary Target hypochlorous acid
Confezionamento
Packaged under inert gas
Attenzione
Toxicity: Standard Handling (A)
Ricostituzione
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Altre note
Xu, Q., et al. 2015. Angew. Chem. Int. Ed.54, 4890.
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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