Lot-specific information can be found on the certificate of analysis (COA). For example, for lot #: 3952706, the specific activity is 3.0 U/mgP, and the pack size is 100 milliunits, which is equivalent to 0.1 units. Therefore, for this specific lot, there is 0.033 mg of protein. In general, for this material, each mg is about 2 to 4 units, so 100 milliunits or 0.1 unit is equivalent to about 0.025 to 0.05 mg amount of protein.
219373
Cathepsin G, Human Neutrophil
Cathepsin G, Human Neutrophil, CAS 107200-92-0, is a purified native cathepsin G. Acts as a potent agonist of human platelet activation leading to their aggregation.
Sinonimo/i:
Cathepsin G, Human Neutrophil
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100 MI.U.
295,00 €
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Cambia visualizzazione
100 MI.U.
295,00 €
About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352202
NACRES:
NA.77
Prodotti consigliati
Origine biologica
human neutrophils
Livello qualitativo
Descrizione
Merck USA index - 14, 1905
Saggio
≥95% (SDS-PAGE)
Stato
lyophilized solid (Salt-free)
Attività specifica
≥2 units/mg protein
Produttore/marchio commerciale
Calbiochem®
Condizioni di stoccaggio
OK to freeze
tecniche
inhibition assay: suitable
Compatibilità
suitable for molecular biology
applicazioni
life science and biopharma
Condizioni di spedizione
ambient
Temperatura di conservazione
−20°C
Informazioni sul gene
human ... CTSG(1511)
Descrizione generale
Research area: Cell Signaling
Cathepsin G is stored in its active form in azurophil granules of neutrophils or monocytes. Its mature form contains one potential glycan-binding site and three disulfide bonds.[1]
Cathepsin G is stored in its active form in azurophil granules of neutrophils or monocytes. Its mature form contains one potential glycan-binding site and three disulfide bonds.[1]
Applicazioni
Azioni biochim/fisiol
This protease cleaves the triple-helix structure of type I collagen in a restricted manner and degrades nonhelical regions of type I and II collagens, fibronectin, aggrecan, and elastin. Additionally, cathepsin G activates ProMMP-3 and -8.[1] Cathepsin G serves as a chemoattractant for human phagocytes and enhances the random motility of T cells. Additionally, it initiates chemotaxis, activates ERK1/2 and p38 MAPK, and facilitates the translocation of PKCζ to the cell membrane, without inducing a detectable calcium flux.[4] Other biological functions of cathepsin G include: ECM components and plasma proteins degradation, bactericidal actions, cleavage of inflammatory mediators, conversion of angiotensin I to angiotensin II, platelet activation and induction of airway submucosal gland secretion.[5]
Attenzione
Toxicity: Standard Handling (A)
Definizione di unità
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-AAPF-pNA per min at 25°C, pH 7.5.
Nota sulla preparazione
Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Reconstitute in 150 mM NaCl, 50 mM sodium acetate buffer, pH 5.5.
Reconstitute in 150 mM NaCl, 50 mM sodium acetate buffer, pH 5.5.
Ricostituzione
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 week at 4°C or for up to 6 months at -70°C.
Reconstitute in 50 mM NaOAc and 150 mM NaCl, pH 5.5.
Altre note
Glusa, E., and Adam C. 2001. Por. J. Pharmacol.133, 422.
Shamamian, P., et al. 2001. J. Cell Physiol.189, 197.
Groutas, W.C., et al. 1993. Biochem. Biophys. Res. Commun.197, 730.
Stone, P.J., et al. 1993. Biochem. Biophys. Res. Commun.197, 130.
Groutas, W.C., et al. 1992. Arch. Biochem. Biophys.294, 144.
Maison, C.M., et al. 1991. J. Immunol.147, 921.
Travis, J. 1988. Am. J. Med.84, 37.
Shamamian, P., et al. 2001. J. Cell Physiol.189, 197.
Groutas, W.C., et al. 1993. Biochem. Biophys. Res. Commun.197, 730.
Stone, P.J., et al. 1993. Biochem. Biophys. Res. Commun.197, 130.
Groutas, W.C., et al. 1992. Arch. Biochem. Biophys.294, 144.
Maison, C.M., et al. 1991. J. Immunol.147, 921.
Travis, J. 1988. Am. J. Med.84, 37.
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Could you please provide the item number for R: 219373? I recently purchased Human Neutrophil Cathepsin G (219373) with a concentration of 100MIU. I'm experiencing difficulty converting it to µg/ml for the required dilution. Can you assist me with this? Thank you.
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