The Foreign Trade Commodity Code is 2934.99.9001.
909629
Azide-A-DSBSO crosslinker
≥95%
Sinonimo/i:
Azide-tagged acid-cleavable disuccinimidyl bissulfoxide, Bis(2,5-dioxopyrrolidin-1-yl) 3,3′-((2-(3-azidopropyl)-2-methyl-1,3-dioxane-5,5-diyl)bis(methylenesulfinyl))dipropionate, Mass spectrometry-cleavable crosslinker for studying protein-protein interations
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About This Item
Formula empirica (notazione di Hill):
C24H33N5O12S2
Numero CAS:
Peso molecolare:
647.68
Numero MDL:
Codice UNSPSC:
12161502
Prezzi e disponibilità al momento non sono disponibili
Prodotti consigliati
Livello qualitativo
Saggio
≥95%
Stato
powder
Disponibilità
available only in USA
Temperatura di conservazione
2-8°C
Applicazioni
Azide-A-DSBSO (azide-tagged, acid-cleavable disuccinimidyl bissulfoxide) Crosslinker is a homobifunctional, membrane-permeable, enrichable disulfoxide-containing crosslinker for analysis of protein-protein interactions (PPIs) through crosslinking mass spectrometry (XL-MS).
Azide-A-DSBSO possesses two N-hydroxysuccinimide (NHS) ester groups for targeting amines, a ∼14 Å spacer length, two symmetrical acid-cleavable C-S bonds, and a central bioorthogonal azide tag. After reacting with lysine (Lys) residues, the azide tag permits selective enrichment of crosslinked proteins or peptides with a biotin-conjugated alkyne probe to enhance their detection. Additionally, the post-cleavage spacer yields tagged peptides for unambiguous identification by collision-induced dissociation in tandem MS.
Azide-A-DSBSO Crosslinker has mapped in vivo PPIs for target protein complexes and whole proteomes from living cells. It will also be a useful proteomics tool in the quest for targeting ″undruggable″ protein targets.
Azide-A-DSBSO possesses two N-hydroxysuccinimide (NHS) ester groups for targeting amines, a ∼14 Å spacer length, two symmetrical acid-cleavable C-S bonds, and a central bioorthogonal azide tag. After reacting with lysine (Lys) residues, the azide tag permits selective enrichment of crosslinked proteins or peptides with a biotin-conjugated alkyne probe to enhance their detection. Additionally, the post-cleavage spacer yields tagged peptides for unambiguous identification by collision-induced dissociation in tandem MS.
Azide-A-DSBSO Crosslinker has mapped in vivo PPIs for target protein complexes and whole proteomes from living cells. It will also be a useful proteomics tool in the quest for targeting ″undruggable″ protein targets.
Altre note
Technology Spotlight: Cross-Linkers for Elucidation of Protein-Protein Interactions
A New in Vivo Cross-linking Mass Spectrometry Platform to Define Protein-Protein Interactions in Living Cells
Synthesis of two new enrichable and MS-cleavable cross-linkers to define protein–protein interactions by mass spectrometry
A New in Vivo Cross-linking Mass Spectrometry Platform to Define Protein-Protein Interactions in Living Cells
Synthesis of two new enrichable and MS-cleavable cross-linkers to define protein–protein interactions by mass spectrometry
Note legali
Subject to US Patent Application #15/275,001 of the Regents of the University of California
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
4.1B - Flammable solid hazardous materials
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Robyn M Kaake et al.
Molecular & cellular proteomics : MCP, 13(12), 3533-3543 (2014-09-26)
Protein-protein interactions (PPIs) are fundamental to the structure and function of protein complexes. Resolving the physical contacts between proteins as they occur in cells is critical to uncovering the molecular details underlying various cellular activities. To advance the study of
Anthony M Burke et al.
Organic & biomolecular chemistry, 13(17), 5030-5037 (2015-04-01)
The cross-linking Mass Spectrometry (XL-MS) technique extracts structural information from protein complexes without requiring highly purified samples, crystallinity, or large amounts of material. However, there are challenges to applying the technique to protein complexes in vitro, and those challenges become
Articoli
Sulfoxide-containing MS-cleavable cross-linkers capture protein-protein interactions in native cellular environments, aiding PPI identification.
Choosing the right crosslinker for your biochemical or bioconjugation research can be challenging. Our selection guide can help you find the perfect match for your applications.
La scelta del reticolante opportuno per studi di biochimica o di bioconiugazione può essere impegnativa. La nostra guida alla scelta può aiutarvi a trovare l'abbinamento perfetto per le vostre applicazioni.
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