FLE250

Firefly Lantern Extract

for ATP determination

• Szinonimák: FLE
• UNSPSC kód: 12352202
• NACRES: NA.81

Tulajdonságok

• Minőségi szint: 200
• Forma: solution
• kiszerelés: pkg of 250 mg dried lanterns
• technika/technikák: toxicology assay: suitable
• tárolási hőmérséklet: −20°C

Leírás

Általános leírás

The bioluminescent organism, Photinus pyralis firefly is the source of firefly lantern extract. The luciferase enzyme is 60 kDa enzyme which lacks prosthetic group and bound metal in its structure. The N-terminal harbors residues crucial for the bioluminescent activity and the C-terminal possess peroxisome targeting motif.

Alkalmazás

Fireflies have been used in several studies of bioluminescence. In particular they may be used in assays to determine toxicity pathways because bioluminescence can quantitatively measure a wide range of biological activities.

Firefly Lantern Extract has been used in the detection of ATP concentrations by bioluminescence flash analysis in seawater samples, bioluminescence assay in river sediments samples, marine sediments and in quantifying extracellular ATP in red blood cells.

Biokémiai/fiziológiai hatások

Firefly Lantern Extract containing luciferase catalyzes oxidative decarboxylation of luciferin (LH2) in the presence of ATP, oxygen and Mg²⁺ to yield a light-emitter oxyluciferin.

Egyéb megjegyzések

Crude luciferin and luciferase

Feloldás

After reconstitution with 25 mL of water, vial will contain 0.06 M potassium phosphate, pH 7.4, and 0.02 M MgSO₄^.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable