B16 melanoma 4A5 Cell Line from mouse

94042254, mouse skin, Fibroblast-like

• UNSPSC kód: 41106514

Tulajdonságok

• product name: B16 melanoma 4A5 Cell Line from mouse, Mouse melanoma, producing melanin, 94042254
• biológiai forrás: mouse skin
• növekedési mód: Adherent
• kariotípus: Not specified
• morfológia: Fibroblast-like
• termékek: Melanin
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Mouse melanoma, melanin producing

Sejtvonal leírása

Derived from a melanoma from the skin of a C57BL/6 strain mouse, showing fibroblast-like characteristics, which produces melanin. Cells may lose ability to produce pigmentation in long term culture.

Alkalmazás

B16 melanoma 4A5 cell line has been used to study the spectral and biological characteristics of copper (II) mesoporphyrinic complexes. It has also been used to evaluate the cytotoxicity of JaZ-30, a matrix metalloproteinase-2 (MMP-2) inhibitor.

Táptalaj

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Egyéb megjegyzések

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