HEL 299

87042207, human lung (embryonic), Fibroblast

• UNSPSC kód: 41106514

Tulajdonságok

• Terméknév: HEL 299, 87042207
• biológiai forrás: human lung (embryonic)
• növekedési mód: Adherent
• kariotípus: 2n = 46, diploid
• morfológia: Fibroblast
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Human embryo lung

Sejtvonal leírása

Derived from embryonic lung tissue of a male in 1965. It is one of the few human diploid lines to have type A glucose-6-phosphate dehydrogenase. Cells have a limited lifespan of 20 serial subcultures from tissue of origin. Ethnicity: Black.

Alkalmazás

HEL 299 has been used to study the antiviral properties of AIC246, a novel anti-human cytomegalovirus compound. It has also been used to study the role of SUMO ligase protein inhibitor of activated STAT1 (PIAS1) in the intrinsic antiviral immune response to herpes simplex virus.

DNS profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 11,12
D16S539: 10,11
D5S818: 11,13
D7S820: 8,11
THO1: 7
TPOX: 8,12
vWA: 16

Táptalaj

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Limited lifespan.

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