NRK-52E

87012902, rat kidney, Epithelial

• UNSPSC kód: 41106514
• biológiai forrás: rat kidney
• növekedési mód: Adherent
• kariotípus: Not specified
• morfológia: Epithelial
• termékek: Not specified
• receptorok: Not specified

Tulajdonságok

• Terméknév: NRK-52E, 87012902
• biológiai forrás: rat kidney
• növekedési mód: Adherent
• kariotípus: Not specified
• morfológia: Epithelial
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Rat kidney epithelial

Sejtvonal leírása

Cloned from the same mixed culture of normal rat kidney cells as NRK-49F, but has epithelial-like morphology. It has distinct growth and transforming characteristics distinct from NRK-49F.

Alkalmazás

NRK-52E has been used to study the effects of altered selenium supply on the expression of endogenous thioredoxin reductase (TrxR).

Táptalaj

DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5-10% Foetal Bovine Serum (FBS) or New Born Calf Serum (NBCS).

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Remove all traces of trypsin from cultures during sub-culturing i.e. spin cells or neutralise with serum.

Egyéb megjegyzések

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