Detroit 551

86082802, human skin (embryonic), Fibroblast

• UNSPSC kód: 41106514
• biológiai forrás: human skin (embryonic)
• növekedési mód: Adherent
• kariotípus: 2n = 46
• morfológia: Fibroblast
• termékek: Not specified
• receptorok: Not specified

Tulajdonságok

• Terméknév: Detroit 551, 86082802
• biológiai forrás: human skin (embryonic)
• növekedési mód: Adherent
• kariotípus: 2n = 46
• morfológia: Fibroblast
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Human Caucasian embryo skin

Sejtvonal leírása

Derived from a skin biopsy of a normal Caucasian female embryo. Cells may be considered normal and may serve as controls for study of cells having mutant chromosomes. Cells support growth of herpes viruses and SV40 virus can induce transformation.

Alkalmazás

Chromosome and virus studies (Normal control): herpesviruses, SV40

DNS profil

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 12,13
D5S818: 12,13
D7S820: 10,12
THO1: 9,9.3
TPOX: 8
vWA: 18

Táptalaj

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan; at most 25 subcultures from original tissue.

Egyéb megjegyzések

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