h1RPE7

9061602, human eye, Polygonal

• UNSPSC kód: 41106514
• biológiai forrás: human eye
• növekedési mód: Adherent
• kariotípus: Not specified
• morfológia: Polygonal
• termékek: Cytokeratins 5 and 8, junctional protein zonula occludens (ZO-1)
• receptorok: Not specified

Tulajdonságok

• Terméknév: h1RPE7, 09061602, from human eye
• biológiai forrás: human eye
• növekedési mód: Adherent
• kariotípus: Not specified
• morfológia: Polygonal
• termékek: Cytokeratins 5 and 8, junctional protein zonula occludens (ZO-1)
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• kiszállítva: dry ice

Leírás

Sejtvonal eredete

An immortalized retinal pigment epithelial cell line

Sejtvonal leírása

The h1RPE7 cell line was derived from primary human retinal pigment epithelial cells obtained from a 50-year-old female Caucasian donor. The primary culture was transfected with a construct encoding SV40 large T antigen that was deleted for the small t antigen along with puromycin resistance gene.

DNS profil

STR-PCR Data:
Amelogenin: X
CSF1PO: 10,13
D13S317: 12
D16S539: 12
D5S818: 9,13
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 16,19

Táptalaj

Hams F10 + 20% Heat-inactivated FCS + 2 mM Glutamine + selection antibiotic 1μg/ml puromycin.

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approx 24hrs.

Egyéb megjegyzések

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Jogi nyilatkozat

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.