C4618

Monoclonal Anti-Cathepsin L antibody produced in mouse

clone CPL33/1, purified from hybridoma cell culture

• Szinonimák: Anti-CATL, Anti-CTSL, Anti-MEP
• MDL-szám: MFCD01321805
• UNSPSC kód: 12352203
• NACRES: NA.41
• konjugátum: unconjugated
• application: ELISA (i); IHC; WB
• klón: CPL33/1, monoclonal
• faj reaktivitás: human
• citations: 8
• technika/technikák: immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 0.1-0.2 μg/mL using total cell extracts of A549 cells

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 200
• konjugátum: unconjugated
• antitest forma: purified immunoglobulin
• antitest terméktípus: primary antibodies
• klón: CPL33/1, monoclonal
• Forma: buffered aqueous solution
• molekulatömeg: antigen ~25 kDa (human cathepsin L); antigen ~42 kDa (human pro cathepsin L)
• faj reaktivitás: human
• koncentráció: ~2 mg/mL
• technika/technikák: immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 0.1-0.2 μg/mL using total cell extracts of A549 cells
• izotípus: IgG1
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... CTSL1(1514)

Leírás

Általános leírás

Anti-Cathepsin L antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma CPL33/1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human procathepsin L.

Monoclonal Anti-Cathepsin L (mouse IgG1 isotype) is derived from the hybridoma CPL33/1 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from BALB/c mice immunized with human procathepsin L.

Egyediség

The antibody recognizes the native and denaturated forms of the protein and does not cross react with human cathepsin V. Monoclonal Anti-Cathepsin L specifically recognizes human cathepsin L (∼ 25 kDa) and procathepsin L (∼ 42 kDa). Anti-Cathepsin L antibody epitope resides within amino acids of human cathepsin L (FYKE).

Immunogén

human procathepsin L. The antibody epitope resides within amino acids 258-261 of human cathepsin L (FYKE).

Alkalmazás

Anti-Cathepsin L antibody has been used for immunoblotting.

Monoclonal Anti-Cathepsin L antibody produced in mouse is suitable for:

• immunohistochemistry
• indirect ELISA
• western blot : 0.1-0.2 μg/mL using total cell extracts of A549 cells

Biokémiai/fiziológiai hatások

Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumor invasion and metastasis.

Inhibition of the enzyme or the proenzyme by low molecular weight inhibitors or by specific antibodies led to a suppression of the invasive capabilities of malignant cells, or a decline in their ability to form tumors in experimental in vivo and in vitro models.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: nwg
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter