PLN70

GenElute^™ Plasmid Miniprep Kit

sufficient for 70 purifications

• Szinonimák: GenElute^™ Plasmid Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute
• UNSPSC kód: 41105501
• NACRES: NA.52

Tulajdonságok

• használat: sufficient for 70 purifications
• Minőségi szint: 200
• környezetbarátabb alternatív termék tulajdonságai: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• technika/technikák: DNA purification: suitable
• környezetbarátabb alternatív kategória: , Aligned
• tárolási hőmérséklet: 15-25°C

Leírás

Általános leírás

The GenElute Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 15 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Alkalmazás

The DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transformation, transcription, conventional, and automated sequencing.

Tulajdonságok és előnyök

• Purified plasmid DNA in less than 30 minutes
• Purify up to 15 μg of plasmid DNA
• No detectable genomic DNA or RNA contamination
• No phenol/chloroform extraction or alcohol precipitation required

Egyéb megjegyzések

For additional information, please see www.sigma-aldrich.com/genelutehp.

Jogi információk

GenElute is a trademark of Sigma-Aldrich Co. LLC

Biztonsági adatok

• Piktogramok: GHS02,GHS08,GHS05,GHS07
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H226,H290,H302,H315,H319,H334,H336
• Óvintézkedésre vonatkozó mondatok: P210 - P233 - P234 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Veszélyességi osztályok: Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Célzott szervek: Central nervous system
• Tárolási osztály kódja: 3 - Flammable liquids