C1359

Calcein AM solution

≥90% purity (HPLC), solution

• Tapasztalati képlet (Hill-képlet): C₄₆H₄₆N₂O₂₃
• CAS-szám: 148504-34-1
• Molekulatömeg: 994.86
• EC-szám: 200-664-3
• MDL-szám: MFCD05861516
• UNSPSC kód: 12171500
• PubChem Substance ID: 24892405
• NACRES: NA.47

Tulajdonságok

• Terméknév: Calcein AM solution, 4 mM in DMSO, ≥90% (HPLC), solution
• Minőségi szint: 100
• Teszt: ≥90% (HPLC)
• Forma: solution
• koncentráció: 4 mM in DMSO
• technika/technikák: titration: suitable
• szín: colorless; white to off-white
• λmax: 491-497 nm
• alkalmazás(ok): diagnostic assay manufacturing; hematology; histology
• tárolási hőmérséklet: −20°C
• SMILES string: CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45
• InChI: 1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3
• Nemzetközi kémiai azonosító kulcs: XKFSBWQWNMZWFA-UHFFFAOYSA-N

Leírás

Alkalmazás

In cells, calcein AM solution has been used in calcein-AM dye transfer assay. It has been used for the staining of cells to study quantification of cell-migration. It has been used to study cytotoxic effects of drugs in cells.

Biokémiai/fiziológiai hatások

Calcein AM is a membrane permeable nonfluorescent molecule. It is converted to fluorescent calcein by intracellular esterases. Calcein has limited membrane permeability. Calcein AM is also a substrate for p-glycoprotein (Pgp). Presence of Pgp results in efflux of calcein AM and thereby decreases cellular fluorescence. On the other hand, presence of Pgp inhibitor increases cellular entry of calcein AM and thereby increases cellular fluorescence. Calcein AM plays an important role in early drug discovery.

Fluorescent cell permeable derivative of calcein.

Fizikai forma

Solution in 0.4% Calcein AM and 99.6% DMSO.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 2
• Lobbanási pont (F): 185.0 °F - closed cup
• Lobbanási pont (C): 85 °C - closed cup
• Egyéni védőeszköz: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)