PICM0RG50

Millicell^® Standing Cell Culture Inserts

pore size 0.4 μm, diam. 30 mm, transparent PTFE membrane, hydrophilic, H 5 mm, size 6 wells, sterile

• Szinonimák: Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm, Millicell-CM, cell culture inserts, organotypic inserts, plate inserts
• UNSPSC kód: 41104923
• eCl@ss: 32011202
• NACRES: NB.22

Tulajdonságok

• material: polystyrene housing; transparent PTFE membrane
• Minőségi szint: 400
• sterilitás: ethylene oxide treated; sterile
• Jellemzők: hydrophilic
• gyártó/kereskedő neve: Millicell^®
• kiszerelés: pkg of Individually blister packaged
• parameter: 50 °C max. temp.
• technika/technikák: cell attachment: suitable; cell culture | mammalian: suitable; cell differentiation: suitable
• H: 5 mm
• Átmérő: 30 mm
• méret: 6 wells
• külső felület: 4.2 cm²
• szín: transparent membrane, when wetted
• matrix: Biopore^™
• pórusméret: 0.4 μm
• kötéstípus: low binding surface
• észlelési módszer: fluorometric
• kiszállítva: ambient

Leírás

Általános leírás

With Millicell inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell inserts make it possible to study both sides of the cell monolayer.

Alkalmazás

• Cell Attachment, 3D Cell Culture, Cell Growth, Cell Differentiation , Immunocytochemistry
• Biopore Membrane (hydrophilic PTFE) is ideal for low protein-binding, live cell viewing, and immunofluorescent applications

Tulajdonságok és előnyök

• For high cell viability and superior study of three dimensional explant structure
• Lower height allows them to fit inside a standard petri dish
• The Biopore (PTFE) membrane provides high viability—for as long as 40 days—and excellent trans-membrane oxygen transport
• The membrane is optically clear and optimized for long-term organotypic explant maintenance

Elkészítési megjegyzés

To ensure proper wetting of the membrane from the basolateral side and tomitigate a chance of bubble formation:

1. Add assay solution or media to the receiver well: 2 mL for 6 well format orequivalent dimensions.
2. With forceps, place the insert into the receiver well at a 30°-45° angle togradually wet it out from the basolateral side.
3. Once edges start to wet out, gently lower the insert until it is fully seatedinto the well.

Jogi információk

BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 10-13 - German Storage Class 10 to 13