MABS1310

Anti-Apolipoprotein A-I Antibody, clone Wt20-7

clone Wt20-7, from mouse

• Szinonimák: Apo-AI, ApoA-I, Apolipoprotein A1
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• klón: Wt20-7, monoclonal
• application: DB; ELISA; ICC; WB
• faj reaktivitás: human
• technika/technikák: ELISA: suitable; dot blot: suitable; immunocytochemistry: suitable; western blot: suitable

Tulajdonságok

• biológiai forrás: mouse
• antitest forma: purified immunoglobulin
• antitest terméktípus: primary antibodies
• klón: Wt20-7, monoclonal
• faj reaktivitás: human
• kiszerelés: antibody small pack of 25 μL
• technika/technikák: ELISA: suitable; dot blot: suitable; immunocytochemistry: suitable; western blot: suitable
• izotípus: IgG1
• NCBI elérési szám: NP_000030
• UniProt elérési szám: P02647
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... APOA1(335)

Leírás

Általános leírás

Apolipoprotein A-I (UniProt: P02647; also known as Apo-AI, ApoA-I, Apolipoprotein A1) is encoded by the APOA1 gene (Gene ID: 335) in human. Apo A-1 is a major protein of plasma high density lipoprotein (HDL) and is also found in chylomicrons. It plays a major role in transporting cholesterol from peripheral tissues to the liver. It is synthesized mainly in the liver with a signal peptide (aa 1-18), which is proteolytically cleaved to generate the mature protein. Apo A-1 also serves as a co-factor for lecithin cholesterolacyltransferase (LCAT) that is involved in the formation of cholesteryl esters. Elevated levels of oxidized Apo A-1 (on Methionine 110 and 136) have been linked to increased risk for coronary artery disease. Mutations in APOA1 gene have also been linked to HDL deficiencies that lead to premature coronary artery disease and a reduction in cellular cholesterol efflux. Mutations in APOA1 gene have also shown to result in amyloidosis with extensive visceral amyloid deposit and in the pathogenesis of amyloid polyneuropathy-nephropathy Iowa type, also known as familial amyloid polyneuropathy type III.

Egyediség

Clone Wt20-7 specifically detects human Apolipoprotein A-I.

Immunogén

GST-tagged full-length human recombinant Apolipoprotein A-I

Alkalmazás

Anti-Apolipoprotein A-I, clone Wt20-7, Cat. No. MABS1310, is a mouse monoclonal antibody that detects human Apolipoprotein A-I and has been tested for use in Dot Blot, ELISA, Immunocytochemistry, and Western Blotting.

ELISA Analysis: A representative lot detected Apolipoprotein A-I in ELISA applications (Kimura, H., et. al. (2017). Sci Rep. 7(1):2988).

Western Blotting Analysis: A representative lot detected Apolipoprotein A-I in Western Blotting applications (Kuwabara, K., et. al. (2015). J Biol Chem. 290(40):24210-21).

Immunocytochemistry Analysis: A representative lot detected Apolipoprotein A-I in Immunocytochemistry applications (Kameyama, H., et. al. (2016). Sci Rep. 6:30391; Kuwabara, K., et. al. (2015). J Biol Chem. 290(40):24210-21).

Dot Blot Analysis: A representative lot detected Apolipoprotein A-I in Dot Blot applications (Kimura, H., et. al. (2017). Sci Rep. 7(1):2988; Kameyama, H., et. al. (2016). Sci Rep. 6:30391; Kuwabara, K., et. al. (2015). J Biol Chem. 290(40):24210-21).

Research Category
Signaling

Minőség

Evaluated by Western Blotting in human fetal liver tissue lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Apolipoprotein A-I in human fetal liver tissue lysate.

Cél megnevezése

~28 kDa observed; 30.78 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Fizikai forma

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Tárolás és stabilitás

Stable for 1 year at 2-8°C from date of receipt.

Egyéb megjegyzések

Concentration: Please refer to lot specific datasheet.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable