ABS233
Anti-PI3 Kinase p85 Antibody, N-SH2 domain
from rabbit, purified by affinity chromatography
Szinonimák:
Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-al
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(4)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
polyclonal
application:
IP
WB
WB
faj reaktivitás:
human, rat
technika/technikák:
immunoprecipitation (IP): suitable
western blot: suitable
western blot: suitable
citations:
15
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
affinity isolated antibody
antitest terméktípus
primary antibodies
klón
polyclonal
tisztítva
affinity chromatography
faj reaktivitás
human, rat
faj reaktivitás (homológia által előrejelzett)
mouse (based on 100% sequence homology), monkey (based on 100% sequence homology), bovine (based on 100% sequence homology)
technika/technikák
immunoprecipitation (IP): suitable
western blot: suitable
NCBI elérési szám
UniProt elérési szám
kiszállítva
wet ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
bovine ... Pik3R1(282307)
human ... PIK3R1(5295)
mouse ... Pik3R1(18708)
rat ... Pik3R1(25513)
rhesus monkey ... Pik3R1(698996)
Általános leírás
Phosphatidylinositol 3-kinase regulatory subunit alpha (UniProt Q63787; also known as Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha) is encoded by the Pik3r1 gene (Gene ID 25513) in rat species. PI3K regulatory subunit alpha catalyzes the phosphorylation of PI(4,5)P2 inositol ring at the 3 position to generate PI(3,4,5)P3, a potent second messenger that mediates survival signaling and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Following ligand binding, tyrosine phosphorylation of growth factor receptors creates docking sites for p85 binding (through its SH2 domains), and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Plays a role in insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Mutations in Pik3r1 gene are known to be associated with insulin resistance.
Egyediség
This polyclonal antibody targets the first SH2 domain region common to human (isoform 1, AS53, p46, p85I), mouse, and rat (isoform p85-alpha, p55-alpha, and p50-alpha) spliced isoforms of PI3 kinase regulatory subunit alpha reported by UniProt (P27986, P26450, Q63787).
Immunogén
Epitope: First SH2 domain.
GST-tagged recombinant fragment corresponding to the first SH2 domain of rat PI3 kinase regulatory subunit alpha.
Alkalmazás
Detect PI3 Kinase regulatory subunit alpha using this rabbit polyclonal Anti-PI3 Kinase p85, N-SH2 domain, Cat. No. ABS233, validated for use in Immunoprecipitation and Western Blotting.
Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated PI3 kinase regulatory subunit alpha isoforms from 200 µg of rat brain tissue lysate.
Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated PI3 kinase regulatory subunit alpha isoforms from 500 µg of Jurkat cell lysate.
Western Blotting Analysis: 2 µg/mL from a representative lot detected PI3 kinase regulatory subunit alpha isoforms in 10 µg of Jurkat cell lysate.
Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated PI3 kinase regulatory subunit alpha isoforms from 500 µg of Jurkat cell lysate.
Western Blotting Analysis: 2 µg/mL from a representative lot detected PI3 kinase regulatory subunit alpha isoforms in 10 µg of Jurkat cell lysate.
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
Minőség
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PI3 kinase regulatory subunit alpha isoforms in 10 µg of rat brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PI3 kinase regulatory subunit alpha isoforms in 10 µg of rat brain tissue lysate.
Cél megnevezése
~85/50 kDa observed. 83.47/53.49/49.97/84.47 kDa (human isoform 1/AS53/p46/p85I; UniProt P27986), 83.39 kDa (mouse; UniProt P26450), 83.40/53.39/50.00 kDa (rat p85-alpha/p55-alpha/p50-alpha isoforms; UniProt Q63787). Uncharacterized bands may be observed in some lysate(s).
Kapcsolódás
Replaces: 06-496
Fizikai forma
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Tárolás és stabilitás
Stable for 1 year at 2-8°C from date of receipt.
Analízis megjegyzés
Control
Jurkat cell lysate
Jurkat cell lysate
Egyéb megjegyzések
Concentration: Please refer to lot specific datasheet.
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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