ABE44

Anti-trimethyl Histone H3 (Lys27) Antibody

from rabbit, purified by affinity chromatography

• Szinonimák: H3K27me3, Histone H3 (tri methyl K27), H3 histone family, member T, histone 3, H3, histone cluster 3, H3, H3K27me3
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• klón: polyclonal
• application: DB; ICC; WB; inhibition assay
• faj reaktivitás: mouse, human
• technika/technikák: dot blot: suitable; immunocytochemistry: suitable; inhibition assay: suitable (peptide); western blot: suitable
• citations: 43

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 100
• antitest forma: affinity isolated antibody
• antitest terméktípus: primary antibodies
• klón: polyclonal
• tisztítva: affinity chromatography
• faj reaktivitás: mouse, human
• technika/technikák: dot blot: suitable; immunocytochemistry: suitable; inhibition assay: suitable (peptide); western blot: suitable
• NCBI elérési szám: NP_003484.1
• UniProt elérési szám: Q16695
• kiszállítva: wet ice
• célzott transzláció utáni módosítás: trimethylation (Lys27)
• Géninformáció: human ... HIST1H3F(8968)

Leírás

Általános leírás

Histone H3 is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 lysine 27 methylation (H3K27) is associated with heterochromatin formation and transcriptional repression.

Egyediség

Broad species cross-reactivity expected.

This antibody recognizes Histone H3 methylated at Lys27.

Immunogén

Branch peptide corresponding to human Histone H3 methylated at Lys27.

Epitope: Lys27

Alkalmazás

Anti-trimethyl Histone H3 (Lys27) Antibody is a rabbit polyclonal antibody for detection of Histone H3 trimethylated on lysine 27( H3K27me3). This purified polyclonal is specificity verified by dot blot (DB), published in peer reviewed journals and validated in WB, ICC, PIA.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in NIH/3T3 cells.
Peptide Inhibition Assay: This antibody peptide blocked on HeLa cell extracts.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Minőség

Evaluated by Western Blot in HeLa acid extract and recombinant Histone H3.

Western Blot Analysis: 0.1 µg/ml of this antibody detected histone H3 in 10 µg of HeLa acid extract .

Cél megnevezése

~ 17 kDa

Fizikai forma

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Tárolás és stabilitás

Stable for 1 year at 2-8°C from date of receipt.

Analízis megjegyzés

Control
HeLa acid extract and recombinant Histone H3

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable