ABE257
Anti-RAD51 Antibody
from rabbit, purified by affinity chromatography
Szinonimák:
DNA repair protein RAD51 homolog 1
Short name=HsRAD51
Short name=hRAD51
Alternative name(s):
RAD51 homolog A
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(3)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
polyclonal
application:
ICC
IP
WB
IP
WB
faj reaktivitás:
rat, human, mouse
technika/technikák:
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunoprecipitation (IP): suitable
western blot: suitable
citations:
35
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
affinity isolated antibody
antitest terméktípus
primary antibodies
klón
polyclonal
tisztítva
affinity chromatography
faj reaktivitás
rat, human, mouse
technika/technikák
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI elérési szám
UniProt elérési szám
kiszállítva
wet ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... RAD51(5888)
Általános leírás
RAD51 is essential for homologous recombination of DNA during repair of double-strand breaks. The N-terminus of RAD51 functions to bind DNA and promote homologous pairing and strand exchange within a regular nucleoprotein filament. In yeast, the Rad51 family of related proteins also includes Rad55, Rad57, and Dmc1. In mammalian cells, seven genes in this family have been identified (HsRAD51, XRCC2, XRCC3, RAD51B/hREC2 and HsDMC1, RAD51C, and RAD51D). RAD51 is predominantly expressed in the testis and thymus but can also be found in the small intestine, placenta, colon, pancreas and ovaries.
Egyediség
Other homologies: Bovine, Equine, and Canine (99% sequence homology).
Immunogén
Histidine-tagged recombinant protein corresponding to human RAD51.
Alkalmazás
Anti-RAD51 Antibody is a Rabbit Polyclonal Antibody for detection of RAD51 also known as DNA repair protein RAD51 homolog 1, HsRAD51 & has been validated in WB, ICC & IP.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected RAD51 in A431 and HeLa cells.
Immunoprecipitation Analysis: 40 µLof Anti-RAD51 immunoprecipitated RAD51 from HeLa RIPA lysate.
Immunoprecipitation Analysis: 40 µLof Anti-RAD51 immunoprecipitated RAD51 from HeLa RIPA lysate.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Minőség
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1:2,500 dilution of this antibody detected RAD51 on 10 µg of HeLa cell lysate.
Western Blot Analysis: 1:2,500 dilution of this antibody detected RAD51 on 10 µg of HeLa cell lysate.
Cél megnevezése
~37 kDa observed. Two isoforms (isoform 1 at 37 kDa and isoform 2 at 26 kDa) produced as a result of alternative splicing are known to exist: .
Kapcsolódás
Replaces: MABE515
Fizikai forma
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Tárolás és stabilitás
Stable for 1 year at 2-8°C from date of receipt.
Analízis megjegyzés
Control
HeLa cell lysate
HeLa cell lysate
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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javasolt
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
The role of DNA damage and repair in decitabine-mediated apoptosis in multiple myeloma.
Maes, K; De Smedt, E; Lemaire, M; De Raeve, H; Menu, E; Van Valckenborgh, E; McClue et al.
Oncotarget null
Shiny Titus et al.
Science translational medicine, 5(172), 172ra21-172ra21 (2013-02-15)
The underlying mechanism behind age-induced wastage of the human ovarian follicle reserve is unknown. We identify impaired ATM (ataxia-telangiectasia mutated)-mediated DNA double-strand break (DSB) repair as a cause of aging in mouse and human oocytes. We show that DSBs accumulate
Tsutomu Shimura et al.
Cell cycle (Georgetown, Tex.), 16(24), 2345-2354 (2017-11-04)
Ionizing radiation (IR) elevates mitochondrial oxidative phosphorylation (OXPHOS) in response to the energy requirement for DNA damage responses. Reactive oxygen species (ROS) released during mitochondrial OXPHOS may cause oxidative damage to mitochondria in irradiated cells. In this paper, we investigated
Thomas Chabot et al.
Cancers, 11(3) (2019-03-27)
Genomic instability through deregulation of DNA repair pathways can initiate cancer and subsequently result in resistance to chemo and radiotherapy. Understanding these biological mechanisms is therefore essential to overcome cancer. RAD51 is the central protein of the Homologous Recombination (HR)
Anastasia Tsvetkova et al.
Oncotarget, 8(38), 64317-64329 (2017-10-04)
At high exposure levels ionizing radiation is a carcinogen. Little is known about how human stem cells, which are known to contribute to tumorigenesis, respond to prolonged radiation exposures. We studied formation of DNA double strand breaks, accessed as γH2AX
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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