71405-M
Rosetta 2 Competent Cell Set - Novagen
Escherichia coli, rod shaped
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
41106202
NACRES:
NA.81
biológiai forrás:
Escherichia coli
növekedési mód:
adherent or suspension
morfológia:
rod shaped
gyártó/kereskedő neve:
Novagen®
Javasolt termékek
Terméknév
Rosetta 2 Competent Cell Set - Novagen,
biológiai forrás
Escherichia coli
Minőségi szint
gyártó/kereskedő neve
Novagen®
tárolási körülmény
OK to freeze
növekedési mód
adherent or suspension
morfológia
rod shaped
technika/technikák
microbiological culture: suitable
kiszállítva
dry ice
tárolási hőmérséklet
−70°C
Related Categories
Általános leírás
Genotypes:
Rosetta 2
F-ompT hsdSB(rB- mB-) gal dcm pRARE2 (CamR)
Rosetta 2(DE3)
F-ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE2 (CamR)
Rosetta 2(DE3)pLysS
F-ompT hsdSB(rB- mB-) gal dcm (DE3) pLysSRARE2 (CamR)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Rosetta 2
F-ompT hsdSB(rB- mB-) gal dcm pRARE2 (CamR)
Rosetta 2(DE3)
F-ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE2 (CamR)
Rosetta 2(DE3)pLysS
F-ompT hsdSB(rB- mB-) gal dcm (DE3) pLysSRARE2 (CamR)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Rosetta 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
Komponensek
•2 x 0.2 mlRosetta 2 Competent Cells
•2 x 0.2 mlRosetta 2(DE3) Competent Cells
•2 x 0.2 mlRosetta 2(DE3)pLysS Competent Cells
•4 x 2 mlSOC Medium
•2 ngTest Plasmid
•2 x 0.2 mlRosetta 2(DE3) Competent Cells
•2 x 0.2 mlRosetta 2(DE3)pLysS Competent Cells
•4 x 2 mlSOC Medium
•2 ngTest Plasmid
Figyelmeztetés
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Jogi információk
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
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