218783

Caspase-1, Human, Recombinant, E. coli

Caspase-1, Human, Recombinant, is expressed in E. coli and is fused at the N-terminus to a His•Tag sequence.

• Szinonimák: IL-1β Converting Enzyme, ICE
• UNSPSC kód: 12352202
• NACRES: NA.77

Tulajdonságok

• rekombináns: expressed in E. coli
• Minőségi szint: 100
• Teszt: ≥90% (SDS-PAGE)
• form: liquid
• specifikus aktivitás: ≥25,000 units/mg protein; ≥50,000 units/mL
• gyártó/kereskedő neve: Calbiochem^®
• tárolási körülmény: OK to freeze; avoid repeated freeze/thaw cycles
• kiszállítva: dry ice
• tárolási hőmérséklet: −70°C

Leírás

Általános leírás

Research area: APOPTOSIS

Caspases are enzymes that belong to the cysteine protease family. In mammals, researchers have discovered 13 caspases. They enhance chemical reactions by targeting specific substances, guided by aspartic acid. These caspases can be further classified into two groups: apoptotic caspases (caspase-2, 3, 6, 7, 8, 9, and 10) and inflammatory caspases (caspase-1, 4, 5, 11, and 12). Apart from inflammatory organs such as the spleen, lymph nodes, and thymus, caspase-1 is also expressed in adipose tissue, liver, and intestine. Recombinant, human caspase-1 fused at the N-terminus to a His•Tag sequence and expressed in E. coli. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. M.W. 10000 and 20000.

Alkalmazás

Caspase-1 enzyme has been used in mediating the proteolysis of pro-IL-1β for regulating the availability and activation of IL-1β.

Biokémiai/fiziológiai hatások

Caspases belong to the cysteine proteases family of enzymes that are responsible for regulating apoptosis and pyroptosis, a programmed necrosis. The activation of caspase-1 in inflammasomes initiates an inflammatory response for fighting infections or cellular damage. Furthermore, caspase-1 activated interleukin-1 beta (IL-1β) and interleukin-18 (IL-18) are related to various metabolic disorders such as obesity, atherosclerosis, impairment of insulin action and/or secretion, cancer, and nonalcoholic fatty liver disease. Caspase-1 deficiency could play a role in promoting the growth of tumor cells in colon, ovarian, and prostate cancers.

Figyelmeztetés

Toxicity: Standard Handling (A)

Egység definíció

One unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-YVAD-pNA (Cat. No. 400025) per min at 30°C, pH 7.4.

Fizikai forma

In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.

Feloldás

Following initial thaw, aliquot and freeze (-70°C).

Egyéb megjegyzések

Thornberry, N.A. 1994. Methods Enzymol. 244, 615.
Thornberry, N.A., et al. 1992. Nature 356, 768.

Jogi információk

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 2