07-523
Anti-trimethyl-Histone H3 (Lys9) Antibody
serum, Upstate®
Szinonimák:
H3K9me3, Histone H3 (tri methyl K9), H3 histone, family 3A
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(3)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
serum
antitest terméktípus
primary antibodies
klón
polyclonal
faj reaktivitás
human
gyártó/kereskedő neve
Upstate®
technika/technikák
dot blot: suitable
inhibition assay: suitable (peptide)
western blot: suitable
izotípus
IgG
NCBI elérési szám
UniProt elérési szám
kiszállítva
dry ice
célzott transzláció utáni módosítás
trimethylation (Lys9)
Géninformáció
human ... H3F3B(3021)
Általános leírás
Histone H3 is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Egyediség
A broad species cross-reactivity is expected.
Recognizes Histone H3 containing trimethyl-lysine 9 and, to a lesser extent, dimethyl-lysine 9. MW ~17 kDa.
Immunogen
BSA-conjugated, synthetic peptide containing the sequence …AR[me3K]S… in which me3K corresponds to trimethyl-lysine 9 of human Histone H3.
Epitope: Trimethyl Lys9
Alkalmazás
Anti-trimethyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of trimethyl-Histone H3 (Lys9) also known as H3K9me3, Histone H3 (tri methyl K9) & has been validated in DB, PIA & WB.
Peptide Inhibition Analysis:
10 μM of a Histone H3 peptide containing trimethyl-lysine 9 abolished detection of Histone H3 by anti-trimethyl Histone H3 (Lys9) in western blot analysis of HeLa acid extracts (Figure B, lane 4). Slight signal reduction was observed with 10 μM of a Histone H3 peptide containing dimethyl-lysine 9.
Peptide Dot Blot Analysis:
A 1:2000 dilution of a previous lot was incubated with serial dilutions of Histone H3 peptides containing various modifications spotted onto PVDF. Slight cross-reactivity with Histone H3 peptide containing dimethyl-lysine 9 was detected.
Beadlyte Histone Peptide Assay:
1:2000-1:6000 dilutions of a previous lot were incubated with Histone H3 peptides containing various modifications conjugated to Luminex microspheres. Slight cross-reactivity with Histone H3 peptides containing dimethyl-lysine 9 and trimethyl-lysine 27 was detected.
10 μM of a Histone H3 peptide containing trimethyl-lysine 9 abolished detection of Histone H3 by anti-trimethyl Histone H3 (Lys9) in western blot analysis of HeLa acid extracts (Figure B, lane 4). Slight signal reduction was observed with 10 μM of a Histone H3 peptide containing dimethyl-lysine 9.
Peptide Dot Blot Analysis:
A 1:2000 dilution of a previous lot was incubated with serial dilutions of Histone H3 peptides containing various modifications spotted onto PVDF. Slight cross-reactivity with Histone H3 peptide containing dimethyl-lysine 9 was detected.
Beadlyte Histone Peptide Assay:
1:2000-1:6000 dilutions of a previous lot were incubated with Histone H3 peptides containing various modifications conjugated to Luminex microspheres. Slight cross-reactivity with Histone H3 peptides containing dimethyl-lysine 9 and trimethyl-lysine 27 was detected.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Minőség
Evaluated by western blot in acid-extracted proteins from HeLa cells.
Western Blot Analysis:
1:500-1:5000 dilution of this antibody detected methylated Histone H3 (Lys9) in acid-extracted proteins from HeLa cells. The antibody did not detect unmethylated recombinant Histone H3 (Catalog # 14-411).
Western Blot Analysis:
1:500-1:5000 dilution of this antibody detected methylated Histone H3 (Lys9) in acid-extracted proteins from HeLa cells. The antibody did not detect unmethylated recombinant Histone H3 (Catalog # 14-411).
Cél megnevezése
~17 kDa
Fizikai forma
Immunodepleted Serum
Rabbit polyclonal antiserum IgG in buffer containing 0.05% sodium azide before the addition of 30% glycerol.
Tárolás és stabilitás
Stable for 1 year at -20ºC from date of receipt.
Analízis megjegyzés
Control
Acid extracted proteins from HeLa cells.
Acid extracted proteins from HeLa cells.
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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