06-720
Anti-HDAC1 Antibody
Upstate®, from rabbit
Szinonimák:
histone deacetylase 1, reduced potassium dependency, yeast homolog-like 1
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(3)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
polyclonal
application:
ICC
IP
WB
activity assay
IP
WB
activity assay
faj reaktivitás:
rat, human, mouse
technika/technikák:
activity assay: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
citations:
86
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
purified immunoglobulin
antitest terméktípus
primary antibodies
klón
polyclonal
faj reaktivitás
rat, human, mouse
kiszerelés
antibody small pack of 25 μg
gyártó/kereskedő neve
Upstate®
technika/technikák
activity assay: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
izotípus
IgG
NCBI elérési szám
UniProt elérési szám
kiszállítva
ambient
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... HDAC1(3065)
mouse ... Hdac1(433759)
rat ... Hdac1(297893)
Általános leírás
Mammalian HADC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all are related to the yeast transcriptional regulator Rpd3p, are identified as histone deacetylases. The deacetylation of histones is associated with transcriptional silencing.
Egyediség
Oher species cross-reactivity not tested.
Recognizes and is specific for HDAC1, Mr 65 kDa. May detects a non-specific protein at ~35 kDa.
Immunogén
6X-His-tagged fragment representing amino acids 53-482 of mouse histone deacetylase 1 (HDAC1).
Alkalmazás
Immunoprecipitation:
5 μg of a previous lot immunoprecipitated HDAC1 from 500 μg of 3T3/A31 RIPA lysate.
Immunocytochemistry:
This antibody has been reported to immunostain HDAC1 in 1% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized 3T3 cells.
HDAC Activity:
This antibody has been reported to immunoprecipitate active HDAC.
5 μg of a previous lot immunoprecipitated HDAC1 from 500 μg of 3T3/A31 RIPA lysate.
Immunocytochemistry:
This antibody has been reported to immunostain HDAC1 in 1% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized 3T3 cells.
HDAC Activity:
This antibody has been reported to immunoprecipitate active HDAC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-HDAC1 Antibody (Rabbit Polyclonal Antibody) validated in EA, ICC, IP, WB to detect HDAC1 also known as histone deacetylase 1.
Minőség
Routinely evaluated by western blot on RIPA lysates from 3T3/A31 cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected HDAC1 in RIPA lysates from 3T3/A31 cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected HDAC1 in RIPA lysates from 3T3/A31 cells.
Cél megnevezése
65 kDa
Fizikai forma
Format: Purified
Protein A chromatography
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, containing 0.05% sodium azide. Frozen at -20ºC.
Tárolás és stabilitás
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analízis megjegyzés
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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javasolt
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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