06-284-AF647

Anti-Nitrotyrosine Antibody Alexa Fluor^™ 647 Conjugate

from rabbit, ALEXA FLUOR^™ 647

• Szinonimák: Nitrotyrosine AlexaFluor^™ 647 Conjugate
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.45

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 100
• konjugátum: ALEXA FLUOR^™ 647
• antitest forma: affinity isolated antibody
• antitest terméktípus: primary antibodies
• klón: polyclonal
• faj reaktivitás: human
• faj reaktivitás (homológia által előrejelzett): all
• technika/technikák: immunocytochemistry: suitable
• kiszállítva: wet ice
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... NOS1(4842)

Leírás

Általános leírás

Nitrotyrosine is produced by the action of reactive nitrogen species, such as peroxinitrite and nitrogen dioxide on tyrosine residues in proteins. Nitrotyrosine is an indicator of cell damage, inflammation, and nitric oxide production. Both free tyrosine and protein bound tyrosine residues are prone to nitration.
In several pathological conditions, oxidative stress is increased resulting in the formation of super oxide and nitric oxide that react to form peroxynitrite (ONOO-), which is a highly reactive molecule. It causes oxidation of proteins and lipids and DNA damage, which results in loss of protein and lipid function and can increase the probability of gene mutations. Higher incidence of protein nitration has been reported in diabetes, atherosclerotic plaques, and rheumatoid arthritis, among others. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected cells and tissues by immunocytochemical techniques using antibodies developed against the nitrotyrosine.

Egyediség

This rabbit polyclonal antibody detects tyrosine nitrated proteins.

Immunogén

Epitope: Nitrated Tyrosine

Nitrated Keyhole Limpet hemocyanain (KLH)

Alkalmazás

Anti-Nitrotyrosine AlexaFluor 647 Conjugate, Cat. No. 06-284-AF647, is a rabbit polyclonal antibody that detects tyrosine nitrated proteins and has been tested for use in Immunocytochemistry.

Research Category
Signaling

Research Sub Category
Developmental Signaling

The unconjugated antibody (Cat. No. 06-284) is shown to be suitable also for Western blotting, immunocytochemistry, immunohistochemistry, and immunoprecipitation.

Minőség

Evaluated by Immunocytochemistry in A431 treated with peroxytnitrite.

Immunocytochemistry Analysis: 1:100 dilution of this antibody detected Nitrotyrosine in A431 cells treated with 5 mM peroxytnitrite.

Fizikai forma

Affinity purified

Purified rabbit polyclonal antibody in buffer containing PBS, pH7.1, and 15 mg/mL BSA with 0.1% sodium azide.

Tárolás és stabilitás

Stable for 1 year at 2-8°C from date of receipt.

Egyéb megjegyzések

Concentration: Please refer to lot specific datasheet.

Jogi információk

ALEXA FLUOR is a trademark of Life Technologies

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable