Ugrás a tartalomra
Merck
Összes fotó(2)

Fontos dokumentumok

04-736

Sigma-Aldrich

Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone SK703, rabbit monoclonal

culture supernatant, clone SK703, Upstate®

Szinonimák:

AKT1 kinase, Protein kinase B, RAC-alpha serine/threonine-protein kinase, rac protein kinase alpha, v-akt murine thymoma viral oncogene homolog 1

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)

About This Item

UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biológiai forrás

rabbit

Minőségi szint

100

antitest forma

culture supernatant

antitest terméktípus

primary antibodies

klón

SK703, monoclonal

faj reaktivitás

mouse, human

gyártó/kereskedő neve

Upstate®

technika/technikák

western blot: suitable

izotípus

IgG

NCBI elérési szám

NM_005163

UniProt elérési szám

P31749

kiszállítva

dry ice

célzott transzláció utáni módosítás

phosphorylation (pSer473)

Géninformáció

human ... AKT1(207)

Általános leírás

The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKBα or RacPK), Akt2 (also designated PKB-β or RacPK-β) and Akt3 (also designated PKB-γ or thyoma viral proto-oncogene 3). Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the 85 kDa subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1- stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases.

Egyediség

Predicted cross-reactivity with rat, bovine, chicken, and Xenopus based on immunogen sequence homology.
Recognizes phosphorylated Akt1/PKBα, Mr 60 kDa. Cross-reactivity to phosphorylated Akt2 and Akt3 likely based on sequence homology.

Immunogen

Epitope: Phosphorylated Ser473
Peptide containing a pSer that corresponds to amino acid position 473 of human Akt1/PKBa

Alkalmazás

Anti-Akt1 Antibody, clone SK703 detects level of phospho-Akt1/PKBα (Ser473) & has been published & validated for use in WB.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling

Minőség

routinely evaluated by immunoblot on lysates from mouse NIH-3T3 fibroblasts treated with PDGF

Cél megnevezése

60kDa

Kapcsolódás

Replaces: 05-736

Fizikai forma

Cultured supernantant in 0.05% sodium azide

Tárolás és stabilitás

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analízis megjegyzés

Control
Untreated and PDGF treated NIH-3T3 fibroblasts.

Jogi információk

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Nem találja a megfelelő terméket?  

Próbálja ki a Termékválasztó eszköz. eszközt

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Yung-Hung Hsieh et al.
Nutrients, 10(9) (2018-09-12)
The anti-inflammatory effect of ginsenoside Rh2 (GRh2) has labeled it as one of the most important ginsenosides. The purpose of this study was to identify the anti-inflammatory and antioxidant effects of GRh2 using a lipopolysaccharide (LPS) challenge lung-injury animal model.
Meiwan Cao et al.
Journal of immunology (Baltimore, Md. : 1950), 198(5), 1887-1899 (2017-01-29)
In myelodysplastic syndromes (MDS), functional defects of neutrophils result in high mortality because of infections; however, the molecular basis remains unclear. We recently found that miR-34a and miR-155 were significantly increased in MDS neutrophils. To clarify the effects of the
Heidi Hynynen et al.
Physiological reports, 8(13), e14474-e14474 (2020-07-10)
Dietary fats are essential for cardiac function. The metabolites of fats known as fatty acids provide most of the energy for cardiac tissue, serve as building blocks for membranes and regulate important signaling cascades. Despite their importance, excess fat intake
Stefan Vallo et al.
BMC research notes, 9(1), 454-454 (2016-09-30)
Systemic chemotherapy with gemcitabine and cisplatin is standard of care for patients with metastatic urothelial bladder cancer. However, resistance formation is common after initial response. The protein Src is known as a proto-oncogene, which is overexpressed in various human cancers.
Jingjing Wu et al.
Molecular medicine reports, 20(4), 3233-3239 (2019-08-23)
Homoharringtonine (HHT) and imatinib have a synergistic effect in the clinical treatment of chronic myeloid leukemia (CML). The purpose of the present study was to explore the underlying mechanisms by which HHT enhanced imatinib sensitivity. K562 CML cells were treated

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

Lépjen kapcsolatba a szaktanácsadással