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Merck

V900893

Sigma-Aldrich

胶原酶 来源于溶组织梭菌

Type IV, Vetec, reagent grade, powder, ≥160 CDU/mg solid

别名:

梭菌肽酶 A

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204

生物源

Clostridium histolyticum

等級

reagent grade

產品線

Vetec

形狀

powder

比活性

≥160 CDU/mg solid

技術

cell culture | mammalian: suitable
single cell analysis: suitable

儲存溫度

−20°C

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相关类别

一般說明

来自溶组织梭菌(Clostridium histolyticum)的胶原酶是一种胶原分解酶。它与胶原蛋白样物质相互作用,将胶原蛋白降解成小分子肽。

應用

来自溶组织梭菌(Clostridium histolyticum)的胶原酶,可用于从许多类型的动物组织中分离细胞。VETEC IV型胶原酶具有较低的胰蛋白酶活性。它适用于消化胰腺中的胰岛细胞。

适用于制备用于测序的单细胞悬浮液。

生化/生理作用

每摩尔胶原蛋白酶被4g原子钙活化。 它被乙二醇-双(β-氨基乙基醚)-N,NN′,N′-四乙酸,β-巯基乙醇、谷胱甘肽、巯基乙酸和8-羟基喹啉抑制。

品質

还含有梭菌蛋白酶、中性蛋白酶和胰蛋白酶活性。

單位定義

一个胶原蛋白消化单元(CDU)从牛跟腱胶原蛋白中释放的肽链量和1.0 μ亮氨酸,5小时,pH7.4,37℃,在钙离子存在下的茚三酮颜色相同。一个 FALGPA水解单元每分钟,25℃,水解1.0 μmole 呋喃基丙烯酰-Leu-Gly-Pro-Ala。一个中性蛋白酶单元水解酪蛋白,产生颜色和1.0 μmole 酪氨酸每5小时,pH7.5,37℃下产生的相同。一个梭菌蛋白酶单元在DTT存在下,pH7.6,25℃,每分钟水解1.0 μmole BAEE。

法律資訊

Vetec is a trademark of Merck KGaA, Darmstadt, Germany

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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