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Merck

H3506

Sigma-Aldrich

透明质酸酶 来源于牛睾丸

Type I-S, lyophilized powder, 400-1000 units/mg solid

别名:

玻璃糖醛酸酶, 玻璃酸酶

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

種類

Type I-S

形狀

lyophilized powder

比活性

400-1000 units/mg solid

分子量

~55 kDa (four subunits of 14 kDa each)

技術

cell culture | mammalian: suitable
single cell analysis: suitable

UniProt登錄號

應用

diagnostic assay manufacturing

運輸包裝

wet ice

儲存溫度

−20°C

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應用

来自牛睾丸的透明质酸酶已用于乳腺组织、心脏组织和卵母细胞的消化以用于细胞的分离。

适用于制备用于测序的单细胞悬液。

生化/生理作用

这些酶可随机裂解透明质酸、软骨素和硫酸软骨素中的 β-N-乙酰己糖胺-[1→4] 糖苷键。
透明质酸酶可降解透明质酸,并已发现可在癌症进展期间会被不适当地调节。

單位定義

在 37 ºC、pH 5.35 条件下,一单位每分钟可降解 0.75 μg 多糖透明质酸(通过比浊吸光度测量(λ: 600nm),与 BSA 复合 45 分钟后测量)。

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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实验方案

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

在测定透明质酸酶活性时,使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下,一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。

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