跳转至内容
Merck
所有图片(1)

文件

P7367

Sigma-Aldrich

糖苷酶F 来源于脑膜脓毒性伊丽莎白菌

BioReagent, ≥95% (SDS-PAGE), for proteomics

别名:

N-糖苷酶F, 糖苷酶F 来源于脑膜炎脓毒性黄杆菌, 糖苷酶F 来源于脑膜脓毒性金黄杆菌, 肽N-糖苷酶

登录查看公司和协议定价
所有图片(1)

About This Item

CAS号:
83534-39-8
EC 号:
3.5.1.52 (BRENDA, IUBMB)
MDL號碼:
MFCD00131222
分類程式碼代碼:
12352204
NACRES:
NA.32

生物源

bacterial (Elizabethkingia meningoseptica)

品質等級

200

共軛

(N-linked)

等級

for proteomics

產品線

BioReagent

化驗

≥95% (SDS-PAGE)

形狀

powder

儲存期限

≥1 weeks at 2‑8 °C (for a reconstituted solution >500 units/ml)
≥1 yr at 2‑8 °C
Solution is stable for at least 3 freeze-thaw cycles

分子量

~36 kDa

濃度

≥300 units/mL
≥50 units/mL

最適pH

~8.6

運輸包裝

wet ice

儲存溫度

2-8°C

正在寻找类似产品? 访问 产品对比指南

相关类别

一般說明

蛋白质组学级糖苷酶F经过从稀磷酸钾缓冲液中充分纯化和冻干，以生产出稳定的产品。 本品不含甘油和其他稳定剂，并且缓冲盐含量非常低。 这种高度纯化的材料非常适用于凝胶、溶液或印迹膜中糖蛋白或糖肽的N-连接去糖基化。

應用

来源于脑膜脓毒性黄杆菌（Elizabethkingia meningoseptica） 的PNGase F已被用于:
  • 寨卡20病毒E蛋白的去N-糖基化
  • 采用表达 CAR 的 COS 细胞评估柯萨奇病毒和腺病毒受体的糖基化
  • 验证 MHC 1 类多肽相关序列 A（MICA）的 N连接糖基化

适用于蛋白质组学和糖生物学；与MALDI-TOF MS分析相容
用于使蛋白质去糖基化。

生化／生理作用

从糖蛋白切割整个聚糖，前提是糖基化天冬酰胺部分在其氨基和羧基末端被多肽链取代。

單位定義

一个酶活性单位是指在37℃、pH 7.5条件下通过SDS-PAGE监测，在1分钟内催化1nM变性核糖核酸酶B释放N-连接寡糖所需的酶量。 糖苷酶F活性的一个Sigma单位相当于1 IUB毫单位。

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H334

防範說明

P261 - P284 - P501

危險分類

Resp. Sens. 1

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Terry Nguyen-Khuong et al.
Glycoconjugate journal, 35(6), 499-509 (2018-11-24)
Analysis of glycans via a porous graphitized carbon liquid chromatography (PGC-LC) coupled with electrospray ionization (tandem) mass spectrometry (ESI-MS(/MS)) is a powerful analytical method in the field of glycomics. Isobaric glycan structures can be identified reliably with the help of
Lars Andresen et al.
Journal of immunology (Baltimore, Md. : 1950), 188(4), 1847-1855 (2012-01-10)
NKG2D ligand surface expression is important for immune recognition of stressed and neotransformed cells. In this study, we show that surface expression of MICA/B and other NKG2D ligands is dependent on N-linked glycosylation. The inhibitor of glycolysis and N-linked glycosylation
Mikkel G Terp et al.
NPJ precision oncology, 5(1), 65-65 (2021-07-17)
EGFR tyrosine kinase inhibitor (TKI) resistance in non-small cell lung cancer (NSCLC) patients is inevitable. Identification of resistance mechanisms and corresponding targeting strategies can lead to more successful later-line treatment in many patients. Using spectrometry-based proteomics, we identified increased fibroblast
Katherine J D Ashbourne Excoffon et al.
Journal of virology, 81(11), 5573-5578 (2007-03-23)
The coxsackievirus and adenovirus receptor (CAR) is both a viral receptor and homophilic adhesion protein. The extracellular portion of CAR consists of two immunoglobulin (Ig)-like domains, each with a consensus sequence for N-glycosylation. We used chemical, genetic, and biochemical studies
Daniel Nouri-Nejad et al.
Molecular biology of the cell, 32(5), 376-390 (2021-01-07)
Pannexin 1 (PANX1) is a glycoprotein that forms large pore channels capable of passing ions and metabolites such as ATP for cellular communication. PANX1 has been implicated in many diseases including breast cancer and melanoma, where inhibition or deletion of
查看所有相关文献

商品

Post Translational Modification

Protein modifications are crucial for disease study. Analysis methods are key.

Strategies for Deglycosylating N-Linked Glycans

Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

实验方案

Mass Spectrometry of Glycans

Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门