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product name
RNase B 糖蛋白标准品 来源于牛胰腺, Proteomics Grade
等級
Proteomics Grade
品質等級
化驗
≥90% (SDS-PAGE)
形狀
lyophilized powder
儲存溫度
−20°C
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI 密鑰
CQOVPNPJLQNMDC-UHFFFAOYSA-N
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應用
RNase B是带有PNGase F的优选底物，用于证明使用SDS-PAGE或MALDI-MS进行N联去糖基化。PNGase F的活性通常使用RNase B通过监测去糖基化后在12%凝胶中明显的迁移率变化来测定。蛋白质组学级RNase B在酶消化和随后的纯化后已被用作N-聚糖的来源。它也被用作糖蛋白标准品。
其他說明
牛胰腺核糖核酸酶 B 是一种糖蛋白，仅含有 N-连接聚糖。它是由单个结构域组成的球状蛋白，与作为非糖基化核心形式的核糖核酸酶A（RNase A）在混合物中以较少成分的形式天然存在。RNase B 在 Asn 34 处含有一个糖基化位点，在该位点有 5-9 个甘露糖残基连接到壳二糖核心，即 Man 5-9 GlcNAc 2 。由于 Asn 34 糖基化的异质性，RNase B 以 5 种糖基化变体形式存在，分子量约为 15 kDa。
品質
RNAse B糖蛋白标准品已经过高度纯化，以去除污染的RNaseA。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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