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T6567

Sigma-Aldrich

胰蛋白酶 来源于猪胰腺

Proteomics Grade, BioReagent, Dimethylated

别名:

Porcine Trypsin, Trypsin for Mass Spectropetry

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About This Item

EC號碼:
232-650-8
MDL號碼:
MFCD01775455
分類程式碼代碼:
12352204
NACRES:
NA.56

生物源

Porcine pancreas

品質等級

200

產品線

BioReagent

溶解度

1 mM HCl: soluble 1 mg/mL, clear, colorless

運輸包裝

wet ice

儲存溫度

2-8°C

相关类别

一般說明

胰蛋白酶是一种丝氨酸蛋白酶，通常在生物化学和生物学中用作重要的酶试剂。这种方法产生了适用于蛋白质组学研究的高度纯化的胰蛋白酶产物。蛋白质组学等级非常适合用于溶液和凝胶胰蛋白酶消化。胰蛋白酶是一种丝氨酸蛋白酶，存在于几种脊椎动物的消化系统中。

應用

猪胰脏胰蛋白酶被应用于以下应用中：
  • 凝胶内蛋白消化及MALDI-TOF质谱分析
  • 电喷雾电离质谱(ESI-MS)分析
  • L. 植物杆菌的表面蛋白质组分析
  • 凝胶过滤，超离心，旋转阴影电子显微镜
  • 质谱

生化／生理作用

由于胰蛋白酶具有高度特异性裂解能力，胰蛋白酶肽数量有限，因此胰蛋白酶通常用于蛋白质组学研究中的肽定位和蛋白质序列研究。它可特异性地水解精氨酸和赖氨酸残基羧基侧的肽键。该酶还具有酯酶和酰胺酶的活性。胰蛋白酶作为一种细胞培养工具。在工业上，它被用来水解致敏蛋白以生产低致敏牛奶。

其他說明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

相關產品

产品编号
说明
价格

象形圖

Health hazardExclamation mark
GHS08,GHS07

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 1

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000259391
SLCP3866
0000259391
SLCP3866
SLCL8231

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Daniela M Remus et al.
Journal of bacteriology, 195(3), 502-509 (2012-11-24)
Sortases are transpeptidases that couple surface proteins to the peptidoglycan of Gram-positive bacteria, and several sortase-dependent proteins (SDPs) have been demonstrated to be crucial for the interactions of pathogenic and nonpathogenic bacteria with their hosts. Here, we studied the role
Hui Zheng et al.
Molecules (Basel, Switzerland), 18(11), 13425-13433 (2013-11-02)
A novel chemoenzymatic one-pot multicomponent synthesis of thiazole derivatives was developed. A series of thiazole derivatives were synthesized with high yields up to 94% under mild enzyme-catalyzed conditions. The blank and control experiments reveal that trypsin from porcine pancreas (PPT)
Biomarkers of acute and chronic pancreatitis
Kaphalia BS
Biomarkers in Toxicology, 279-289 (2014)
Masayuki Shimoda et al.
Cell transplantation, 21(2-3), 465-471 (2012-07-17)
Porcine islets are considered to be a promising resource for xenotransplantation. However, it is difficult to isolate porcine islets because of the marked fragility and rapid dissociation. Endogenous trypsin is one of the main factors to damage islets during the
Pauline Floch et al.
Gut pathogens, 6, 20-20 (2014-07-06)
A gamma-glutamyl transpeptidase (GGT) is produced by up to 31% of strains of Campylobacter jejuni isolates. C. jejuni GGT is close to Helicobacter pylori GGT suggesting a conserved activity but unlike the latter, C. jejuni GGT has not been studied
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化学处理的重组胰蛋白酶在蛋白质组学和蛋白质表征分析中的应用评测

化学处理的重组胰蛋白酶在蛋白质组学和蛋白质表征分析中的应用评测

Better Protease Detection | High-Sensitivity Protease Detection Assay

Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.

Improving Glycoform Coverage for Mucin Preparations

Pretreatment with Mucinase StcE increases glycopeptide identification from mucin samples, enhancing sample preparation efficiency for glycopeptide analysis.

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实验方案

Trypsin Assay Procedure (EC 3.4.21.4)

Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.

胰蛋白酶（EC 3.4.21.4）的酶学测定方案

本方案适用于使用Nα-苯甲酰基-L-精氨酸乙酯（BAEE）作为底物测定胰蛋白酶活性。本方案是基于连续分光光度速率测定（A253，光程= 1cm）方案：

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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