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應用
来自金黄色葡萄球菌V8的内切蛋白酶Glu-C已被用于：
- 获得S层蛋白（S-layer protein）的蛋白酶裂解片段（来自嗜热脂肪芽孢杆菌ATCC 12980）以进行亲和力研究。
- 酶切天然VSTx-3（电压传感器毒素3）肽以测定其序列。
- 重组纯化PimA（磷脂酰肌醇甘露糖基转移酶）的限制性蛋白水解。
- 消化糖化血红蛋白，用于同位素稀释液体色谱-串联质谱分析。
生化／生理作用
来自 Staphylococcus aureus菌株V8的内切蛋白酶Glu-C是一种丝氨酸蛋白酶，能够水解谷氨酸和天冬氨酸残基的羧基端肽键。Glu-C特异性取决于缓冲液和pH以及可能的切割位点周围的结构。在乙酸铵（pH4.0）或碳酸氢铵（pH7.8），酶偏爱切割谷氨酰键，但是，在磷酸缓冲液（pH7.8）中，Glu-C会切割在其他位点。Glu-C被报道在0.2% SDS（十二烷基硫酸纳）和4.0M尿素中有活性。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1 - Skin Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
其他客户在看
X. Wang et al.
Plant physiology, 111(2), 441-445 (1996-06-01)
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and a 66-kD protein were co-purified from solubilized microsomal preparations of the green alga Botryococcus braunii by Green A agarose, sucrose density gradient, MonoQ, and gel filtration. The 66-kD protein remained intact after 6 M urea treatment
The abalone egg vitelline envelope receptor for sperm lysin is a giant multivalent molecule.
Swanson, W.J., and Vacquier, V.D.
Proceedings of the National Academy of Sciences of the USA, 94(13), 6724-6729 (1999)
M Jarosch et al.
Microbiology (Reading, England), 146 ( Pt 2), 273-281 (2000-03-09)
The cell surface of Bacillus stearothermophilus ATCC 12980 is completely covered with an oblique S-layer lattice. To investigate sequence identities and a common structure-function relationship in S-layer proteins of different B. stearothermophilus wild-type strains, the nucleotide sequence encoding the S-layer
The Protein Protocols Handbook (1996)
Kenneth AW
Human Genome Methods (1997)
实验方案
An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).
基于优化的 LC-MS/MS 工作流程，通过过滤辅助样品制备 (FASP) 对阿达木单抗 (Humira) 绘制低伪影胰蛋白酶酶解和肽图谱。
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