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Merck

MITOISO1

Sigma-Aldrich

线粒体分离试剂盒

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

别名:

Mitochondria Purification Kit

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About This Item

EC號碼:
分類程式碼代碼:
12352200
NACRES:
NA.32

品質等級

用途

sufficient for 10-20 g (animal tissue)
sufficient for 50 assays (2 mL)

儲存期限

2 yr

技術

fractionation: suitable

運輸包裝

dry ice

儲存溫度

−20°C

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應用

线粒体分离试剂盒可简单、快速地分离动物组织中富集的线粒体组分,并检测线粒体内膜的电化学质子梯度(ΔΨ)。用于线粒体介导的细胞凋亡研究。
线粒体分离试剂盒已用于:
  • 分离线粒体和胞浆组分,用于测定支链氨基酸转氨酶(BCAT)活性
  • 分离肾小球和心脏线粒体
  • 分离脑损伤样品中的线粒体,检测线粒体跨膜电位

特點和優勢

  • 特制提取剂和可靠实验方案适合少量分离——在离心管中即可分离富集完整线粒体组分。
  • 获得完整有活性的线粒体——所得线粒体适用于细胞凋亡、氧化应激等体外分析研究。
  • 提供可确定内膜完整性的染色剂和实验方案——无需购买其他试剂,即可确定线粒体内膜完整性。
  • 适配细胞色素C氧化酶检测试剂盒——可轻松确定线粒体外膜完整性。

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • T9201Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solid化学品安全说明书

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

10 - Combustible liquids


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商品

亚细胞组分离心分离是一种常用技术,广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同,因此通过离心,可从温和均质的样品中轻松分离和纯化细胞器组分。

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。

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