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Merck

MITOISO3

Sigma-Aldrich

酵母线粒体分离试剂盒

sufficient for 40 applications (using 20 OD culture preparations), isolation of an enriched mitochondrial fraction from yeast cells

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About This Item

UNSPSC代码:
12352200
NACRES:
NA.32

质量水平

用途

sufficient for 40 applications (using 20 OD culture preparations)

储存温度

−20°C

一般描述

作为真核细胞能产生能量的地点,线粒体的特征是双层膜结构:外膜和折叠的内膜。分离所得的线粒体可用作进行体外研究的工具,如呼吸和能量生成研究,凋亡、mtDNA和mtRNA、以及线粒体蛋白谱分析。

应用

该试剂盒包含通过溶壁酶裂解酵母细胞壁(原生质球形成),然后进行细胞膜裂解/破碎和线粒体分离所需的所有试剂。另外,该试剂盒包括用于蛋白质组学研究的线粒体蛋白质谱分析提取缓冲液和用于完整线粒体的存储缓冲液。酵母线粒体分离试剂盒已被用于线粒体的分离和提取。

特点和优势

• 该试剂盒具有快速、灵敏、易用、特异性强的特征
• 该试剂盒含有通过溶细胞酶(lyticase)(微球形式)轻松、快速地溶解细胞壁,以及之后的细胞膜裂解/破坏及线粒体分离所需的一切试剂。
• 该试剂盒为细胞膜破碎提供了两种替代方法。 第一种方法是降解,第二种方法是均质化。
• 该试剂盒包含一份可用于线粒体蛋白谱分析的提取缓冲液,可供蛋白质组研究使用
• 该试剂盒包含用于完整线粒体的储液。
• 所含的试剂足够进行40次20 OD培养物的处理
• 该试剂盒已在Saccharomyces cerevisiaePichia pastorisSchizosaccharomyces pombe上进行过测试

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • L2524Lyticase from Arthrobacter luteus, lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret化学品安全说明书

  • P8215Protease Inhibitor Cocktail, for use with fungal and yeast extracts, DMSO solution化学品安全说明书

  • C0356Protein Extraction Reagent Type 4化学品安全说明书

警示用语:

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 2

储存分类代码

10 - Combustible liquids


历史批次信息供参考:

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其他客户在看

Christian Streng et al.
The EMBO journal, 40(17), e108083-e108083 (2021-07-14)
Mitochondria are essential organelles because of their function in energy conservation. Here, we show an involvement of mitochondria in phytochrome-dependent light sensing in fungi. Phytochrome photoreceptors are found in plants, bacteria, and fungi and contain a linear, heme-derived tetrapyrrole as
María Guirola et al.
The Biochemical journal, 432(3), 595-605 (2010-09-23)
The Saccharomyces cerevisiae gene PIF1 encodes a conserved eukaryotic DNA helicase required for both mitochondrial and nuclear DNA integrity. Our previous work revealed that a pif1Δ strain is tolerant to zinc overload. In the present study we demonstrate that this
Panos Oikonomou et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(43), 26710-26718 (2020-10-11)
Large-scale proteomic methods are essential for the functional characterization of proteins in their native cellular context. However, proteomics has lagged far behind genomic approaches in scalability, standardization, and cost. Here, we introduce in vivo mRNA display, a technology that converts
Yeast cells expressing the human mitochondrial DNA polymerase reveal correlations between polymerase fidelity and human disease progression
Qian Y, et al.
The Journal of Biological Chemistry (2017)
Erika Steele et al.
Microbial cell factories, 20(1), 138-138 (2021-07-21)
Myo-Inositol Phosphate Synthase (MIP) catalyzes the conversion of glucose 6- phosphate into inositol phosphate, an essential nutrient and cell signaling molecule. Data obtained, first in bovine brain and later in plants, established MIP expression in organelles and in extracellular environments.

商品

氧化应激,在一部分上,是由多种细胞过程产生的活性氧介导,并由诸如酶清除剂或抗氧化剂调节剂等细胞抗氧化剂机制所控制的。自由基,如活性氧,会通过细胞引起细胞损伤。

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

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