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Merck
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M4530

Sigma-Aldrich

培养基 199

With Earle′s salts, ʟ-glutamine and sodium bicarbonate, sterile liquid, suitable for cell culture

别名:

M199 Medium, TCM 199

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

product name

培养基 199, With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

NaHCO3: yes
L-glutamine: yes
HEPES: no
Earle’s salts (5% CO2): yes
phenol red: yes
sodium pyruvate: no

運輸包裝

ambient

儲存溫度

2-8°C

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相关类别

一般說明

培养基199是维生素、氨基酸和其他因素的组合，最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性，特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。
按照 Morgan 公布的原始 M 199 配方配制的完全液体培养基。

應用

培养基199已用于：
  • 作为悬浮培养基维持原代神经元细胞生长
  • 作为胶原蛋白凝胶组分离体培养胚心
  • 在慢速冷冻处理中，维持卵巢组织切片活性

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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商品

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

钙在细胞培养中的应用

钙是一种离子稳定的二价阳离子，在细胞培养基中具有有益和有毒的双重属性，可作为一系列细胞培养基的组成部分。

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Medium 199 (M199) Formulation

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

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