跳转至内容
Merck

I9278

Sigma-Aldrich

胰岛素 溶液 人

sterile-filtered, BioXtra, suitable for cell culture

登录查看公司和协议定价


About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
12352209
NACRES:
NA.77

無菌

sterile-filtered

產品線

BioXtra

形狀

solution

濃度

9.5-11.5 mg/mL

技術

cell culture | mammalian: suitable

雜質

≤50 EU/mL endotoxin

pH值

7.9-8.5

UniProt登錄號

儲存溫度

2-8°C

InChI 密鑰

PBGKTOXHQIOBKM-FHFVDXKLSA-N

基因資訊

human ... INS(3630)

一般說明

胰岛素是由胰岛β-细胞分泌的双链多肽激素。其分子量为~5800 Da。α链和β链是由两个链间二硫键所连接的。α链含有一个链间二硫键。

應用

人胰岛素溶液可作为添加剂用于Dulbecco′改良eagle′培养基(DMEM)培养上皮细胞系和人子宮內膜细胞系,用于最低必需培养基培养人乳腺癌细胞系,用于培养基199培养正常卵巢细胞系。
人胰岛素溶液用于细胞培养的浓度建议为0.5-1 mL/L培养基。
人胰岛素溶液可用于小鼠和大鼠的胰岛素耐受试验。它还可用于人胚胎干细胞(hESC)的体外培养和hMSC(人间充质基质细胞)的成脂分化。

生化/生理作用

胰岛素在各种细胞过程中起作用,包括促进细胞生长、调节细胞周期、促进葡萄糖摄取。常用作哺乳动物细胞无血清培养基的蛋白添加剂,促进并维持哺乳动物细胞的最优生长。

其他說明

InChI:1S/C257H383N65O77S6/c1-29-131(23)205(313-193(339)104-259)252(393)317-204(130(21)22)248(389)288-159(75-82-200(349)350)217(358)282-156(71-78-189(263)335)221(362)308-183-116-403-404-117-184-243(384)305-178(111-324)240(381)294-162(88-123(7)8)225(366)295-168(95-140-53-61-146(329)62-54-140)228(369)283-154(69-76-187(261)333)218(359)290-161(87-122(5)6)223(364)285-158(74-81-199(347)348)220(361)302-174(101-190(264)336)235(376)298-170(97-142-57-65-148(331)66-58-142)231(372)309-182(242(383)304-176(255(396)397)103-192(266)338)115-402-401-114-181(214(355)273-107-194(340)278-153(72-79-197(343)344)216(357)281-151(51-42-84-271-257(267)268)212(353)272-108-195(341)279-166(93-138-46-36-32-37-47-138)227(368)297-167(94-139-48-38-33-39-49-139)230(371)299-171(98-143-59-67-149(332)68-60-143)238(379)320-208(135(27)327)254(395)322-85-43-52-186(322)246(387)286-152(50-40-41-83-258)222(363)321-209(136(28)328)256(398)399)311-250(391)203(129(19)20)316-236(377)164(90-125(11)12)292-229(370)169(96-141-55-63-147(330)64-56-141)296-224(365)160(86-121(3)4)289-210(351)133(25)277-215(356)157(73-80-198(345)346)287-247(388)202(128(17)18)315-237(378)165(91-126(13)14)293-233(374)173(100-145-106-270-120-276-145)301-239(380)177(110-323)280-196(342)109-274-213(354)180(113-400-405-118-185(310-244(183)385)245(386)319-207(134(26)326)253(394)306-179(112-325)241(382)318-206(132(24)30-2)251(392)312-184)307-226(367)163(89-124(9)10)291-232(373)172(99-144-105-269-119-275-144)300-219(360)155(70-77-188(262)334)284-234(375)175(102-191(265)337)303-249(390)201(127(15)16)314-211(352)150(260)92-137-44-34-31-35-45-137/h31-39,44-49,53-68,105-106,119-136,150-186,201-209,323-332H,29-30,40-43,50-52,69-104,107-118,258-260H2,1-28H3,(H2,261,333)(H2,262,334)(H2,263,335)(H2,264,336)(H2,265,337)(H2,266,338)(H,269,275)(H,270,276)(H,272,353)(H,273,355)(H,274,354)(H,277,356)(H,278,340)(H,279,341)(H,280,342)(H,281,357)(H,282,358)(H,283,369)(H,284,375)(H,285,364)(H,286,387)(H,287,388)(H,288,389)(H,289,351)(H,290,359)(H,291,373)(H,292,370)(H,293,374)(H,294,381)(H,295,366)(H,296,365)(H,297,368)(H,298,376)(H,299,371)(H,300,360)(H,301,380)(H,302,361)(H,303,390)(H,304,383)(H,305,384)(H,306,394)(H,307,367)(H,308,362)(H,309,372)(H,310,385)(H,311,391)(H,312,392)(H,313,339)(H,314,352)(H,315,378)(H,316,377)(H,317,393)(H,318,382)(H,319,386)(H,320,379)(H,321,363)(H,343,344)(H,345,346)(H,347,348)(H,349,350)(H,396,397)(H,398,399)(H4,267,268,271)/t131-,132-,133-,134+,135+,136+,150-,151-,152-,153-,154-,155-,156-,157-,158-,159-,160-,161-,162-,163-,164-,165-,166-,167-,168-,169-,170-,171-,172-,173-,174-,175-,176-,177-,178-,179-,180-,181-,182-,183-,184-,185-,186-,201-,202-,203-,204-,205-,206-,207-,208-,209-/m0/s1

外觀

2-8 °C保存

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Farinaz Ghodrati et al.
Scientific reports, 8(1), 8654-8654 (2018-06-07)
At times, it can be difficult to discern if a lack of overlap in reported interactions for a protein-of-interest reflects differences in methodology or biology. In such instances, systematic analyses of protein-protein networks across diverse paradigms can provide valuable insights.
E Comisso et al.
Oncogene, 36(30), 4253-4266 (2017-03-21)
OCT4 (Octamer-binding transcription factor 4) is essential for embryonic stem cell self-renewal. Here we show that OCT4 increases the aggressiveness of high-grade serous ovarian cancer (HG-SOC) by inactivating the Retinoblastoma tumor suppressor pathway and enhancing mitotic stability in cancer cells.
Early cardiac changes in a rat model of prediabetes: brain natriuretic peptide overexpression seems to be the best marker.
Nunes S, et al.
Cardiovascular Diabetology, 12, 44-44 (2013)
Genetic stability of bone marrow-derived human mesenchymal stromal cells in the Quantum System.
Jones M et al.
Cytotherapy, 15, 1323-1323 (2013)
Amer Mahmood et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 25(6), 1216-1233 (2010-03-05)
Directing differentiation of human embryonic stem cells (hESCs) into specific cell types using an easy and reproducible protocol is a prerequisite for the clinical use of hESCs in regenerative-medicine procedures. Here, we report a protocol for directing the differentiation of

实验方案

Discover our collection of primary human hepatic Kupffer cells and protocol for thawing, plating, and growing Kupffer cells. Find the formulation for Kupffer culture maintenance media.

Discover our primary human hepatocytes and plating and maintenance media formulations. Explore our protocol for plateable primary hepatocyte cell culture.

Discover our protocol for thawing, plating, and forming hepatocyte spheroids from human spheroid-qualified plateable hepatocytes. Find the formulation for hepatocyte spheroid maintenance media.

相关内容

Explore our protocol for suspension primary hepatocyte cell culture. Discover our primary human hepatocytes and plating and maintenance media formulations.

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门