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品質等級
形狀
powder
技術
cell culture | mammalian: suitable
成分
L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no
運輸包裝
ambient
儲存溫度
2-8°C
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一般說明
培养基199是维生素、氨基酸和其他因素的组合,最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性,特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。
應用
培养基199已用于:
- 培养原代人脐静脉内皮细胞(HUVEC),以用于细胞活力检测
- 冲洗小母猪子宫角,从子宫恢复第12天妊娠
- 培养腹膜细胞和永生化人间皮细胞(MeT-5A和ATCC-CRL-9444)
數量
配制成每升培养基中含 9.4 克粉末。
重構
补充0.1克/升左旋谷氨酰胺、2.2克/升碳酸氢钠。
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产品编号
说明
价格
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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商品
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
实验方案
粉末培养基及盐混合物极具吸湿性,应注意防潮。
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