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Merck
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文件

G7654

Sigma-Aldrich

凝胶上样液

for NA electrophoresis, solution

别名:

DNA Gel Loading Solution, Gel Loading Buffer

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About This Item

分類程式碼代碼:
12161703
NACRES:
NA.25

一般說明

凝胶上样溶液在电泳期间可用作跟踪染料。染料具有轻微的负电荷，并且与DNA的迁移方向相同，从而使得用户可以监测分子在凝胶中移动的进程。迁移速率随凝胶组成而变化。上样前用样品进行1：6稀释。

應用

适用于琼脂糖或非变性聚丙烯酰胺凝胶电泳（PAGE），该电泳步骤可能是Northern和Southern印迹杂交过程的一部分。

成分

凝胶上样缓冲液含有0.25％溴酚蓝、0.25％二甲苯蓝和40％蔗糖。

其他說明

预期条带迁移如下：
在聚丙烯酰胺凝胶上，二甲苯氰基可与大约450-460bp的DNA共迁移，而溴酚蓝与15-100bp的DNA共迁移。在0.5-1.4％琼脂糖凝胶上，二甲苯氰基可与4kb dsDNA共迁移，而溴酚蓝则与300bp dsDNA共迁移。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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We have characterized an early series of 5,6-bridged dioxinoquinolones which behaved strikingly different from typical quinolones. The 5,6-bridged dioxinoquinolones inhibited Escherichia coli DNA gyrase supercoiling activity but, unlike typical quinolones, failed to stimulate gyrase-dependent cleavable complex formation. Analogous unsubstituted compounds
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While screening Le(a+b+)Polynesian DNA samples for a candidate Se(w) allele, a point mutation (C571-->T) resulting in a new stop codon (Arg191-->stop) in the alpha(1,2)fucosyltransferase gene (FUT2) was identified. This point mutation resulted in the gaining of a new restriction enzyme
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The apoE gene exhibits two common polymorphisms that have been associated with both coronary artery disease and Alzheimer's disease. The polymorphisms create the three allelic isoforms E2, E3, and E4 which are encoded by Cys;-Cys, Cys;-Arg, and Arg;-Arg at amino
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We present a protocol for reliably detecting DNA copy number aberrations in a single human cell. Multiple displacement-amplified DNAs of a cell are hybridized to a 3,000-bacterial artificial chromosome (BAC) array and to an Affymetrix 250,000 (250K)-SNP array. Subsequent copy
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