推荐产品
產品線
Duolink®
技術
proximity ligation assay: suitable
螢光
λex 554 nm; λem 576 nm (orange) (Cyanine 3; Zeiss Filter set 20)
適合性
suitable for fluorescence
應用
Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.
Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Mouse PLUS and Anti-Goat MINUS), orange detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Mouse PLUS and Anti-Goat MINUS), orange detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
The Duolink® In Situ Orange Starter Kit Mouse/Goat requires one primary antibody from mouse and one primary antibody from goat. Orange fluorescence detection reagents are often used with Cyanine 3 filter.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
The Duolink® In Situ Orange Starter Kit Mouse/Goat requires one primary antibody from mouse and one primary antibody from goat. Orange fluorescence detection reagents are often used with Cyanine 3 filter.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
特點和優勢
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
法律資訊
Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- DUO92006Duolink® In Situ PLA® Probe Anti-Goat MINUS, Affinity purified Donkey anti-Goat IgG (H+L)化学品安全说明书
- DUO92001Duolink® In Situ PLA® Probe Anti-Mouse PLUS化学品安全说明书
- DUO92007Duolink® In Situ Detection Reagents Orange化学品安全说明书
- DUO82049Duolink® In Situ Wash Buffers, Fluorescence化学品安全说明书
- DUO82040Duolink® In Situ Mounting Medium with DAPI化学品安全说明书
訊號詞
Danger
危險分類
Aquatic Chronic 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1
儲存類別代碼
8A - Combustible corrosive hazardous materials
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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商品
Duolink®邻位连接技术用于研究神经元相互作用，促进神经科学研究。
Duolink® proximity ligation assay used to study neuron interactions furthering neuroscience research.
本实验方案描述了如何对细胞和组织样品中的蛋白质进行免疫荧光检测。
Support information including tips and tricks, frequently asked questions, and basic troubleshooting.
实验方案
This page details the Duolink® In Situ Short Protocol for fluorescence detection
This protocol describes how to perform immunofluorescent detection of proteins in cells and tissue.
本页详细介绍了Duolink®原位精简实验方案
相关内容
Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay
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