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一般說明
该试剂盒使用荧光染料bisBenzimide(Hoechst 33258)对DNA进行定量所需的所有试剂。该试剂盒可以高灵敏、快速、准确地测定10ng/ml至5μg/ml的双链DNA,即使存在RNA和蛋白质的情况下也是如此。荧光测定法是一种灵敏度高、简单易行的DNA定量方法。荧光染料bisBenzimide(Hoechst 33258)主要结合双链DNA(dsDNA)小沟内的AT序列,专门用于量化DNA的毫微克量(10 ng/ml至10 mg/ml)。当在360 nm激发时,DNA环境下染料在460 nm处的荧光发射显著增加。
應用
DNA定量试剂盒,荧光法已用于测量DNA浓度/含量:
- 新生大鼠心室肌细胞(NRVM)
- 在人间充质干细胞(hMSC)/样品中,进行相对碱性磷酸酶(ALP)量标准化
- 小鼠胚胎成纤维细胞(NIH3T3细胞)
特點和優勢
- 专用于ng级别DNA的定量
- 适用于纯化制备物,和纯化含有RNA和蛋白质的粗提取物中的DNA
仅试剂盒组分
产品编号
说明
- Detailed technical bulletin 1 ea
相關產品
儲存類別代碼
10 - Combustible liquids
历史批次信息供参考:
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