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一般說明
杜氏(Dulbecco′s)磷酸盐缓冲液(DPBS)是在组织培养中广泛使用的平衡盐溶液(BSS)。
應用
在制备和连续转移过程中,可使用D-PBS清洗细胞。
杜氏磷酸盐缓冲液已用于:
- 作为模拟生理溶液成分,体外研究Fe–20Mn–1.2C合金的降解行为
- 溶解朝鲜当归(Angelica gigas)粉末进行微囊化
- 在细胞培养物处理、愈伤试验、transwell试验和凋亡试验中用于清洗细胞
- 洗涤羊膜用于细胞分析
生化/生理作用
杜氏磷酸盐缓冲液可维持细胞渗透压平衡,作为灌洗、运输和稀释用液。它提供正常细胞代谢必需的水和大量无机离子。混入葡萄糖等糖后,这种缓冲盐可提供细胞代谢所需的主要能量。它的缓冲能力可使培养基维持在生理pH范围内。
數量
按照每升培养基9.6克粉末配制。
其他說明
该D-PBS配方中没有Ca或Mg。也可提供加入Ca和Mg的配方
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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